592 METHODS AND FORMULAS MS 33.41 



33.41 Foot 1924 11284, 9:778 



REAGENTS REQUIRED: A. ADS 12.2 Lugol (1905); B. 1% sodium thiosulfate; C. 0.25% 

 potassium permanganate; D. 5% oxalic acid; E. 2% silver nitrate; F. MS 33.1 Foot 

 1924; G. 2% formaldehyde; H. 1% gold chloride; /. DS 11.121 Weigert 1903; J. DS 

 12.221 Weigert 1904 

 method: [G m sections of F 3700.0010 Zenker 1894 material] -^ water -^ A, 5 mins. -^ B. 

 3-^ min. —> C, 5 mins. -^ D, 15-30 mins. -> wash — > E, 48 hrs. — > rinse -^ F, 30 mins, 

 -^ rinse -^ G, 30 mins. ^ i/. 1 hr. -^ wash ^ /, 1 min. — > tap water, till blue — > /, 

 30 sees. -^ balsam, via usual reagents 



33.41 Foot 1927a 1887a, 4:136 



reagents required: A, B, C, D, as MS 33.41 Foot 1924; E. AMS 11.1 Foot 1927; F. 

 0.1% ammonia; G. MS 33.1 Foot 1927a; H. 8% neutral formaldehyde; /. AMS 22.1 

 Foot 1927; /. 5% sodium thiosulfate 

 method: [material as for MS 33.41 Foot 1924] -^ A -> B --^ C --^ D, as MS 33.41 Foot 

 1924^^ water— > E, 15 mins. at 37°C. -^ F, 30 sees. — * rinse —+ G, 5 mins. in each of 

 2 changes —* wash —> H, 3 mins. — > 7, 3 mins. — > rinse — > J", 3 mins. — > wash -^ bal- 

 sam, via usual reagents 



33.41 Foot 1927b 1887a, 4:212 



reagents required: A, B, C, D as MS 33.41 Foot 1924; E. Foot MS 33.1 1927b; F. 8% 



neutral formaldehyde; G. 0.2% gold chloride; H. 5%, sodium thiosulfate; I. DS 11.123 



Harris 1900; J. DS 12.221 van Gieson 1890 

 method: [material as for AIMS 33.41 Foot 1924]-^ A ^ /? -^ C -> D, as AMS 33.41 



Foot 1924 -^ water -^ E, 15 mins. at 45°C. -^ wash -* F, 2 mins. — » rinse -^ (?, 2 



mins. -^ H, 2 mins. — » wash — ^ 7, 3-5 mins. -^ tap water, till blue — > J, 45 sees. — > 



balsam, via usual reagents 



33.41 Gluck 1938 te.^f. 1939 Foot 4349, 19:169 



reagents required: A. ADS 12.2 Gram 1880; 5. 5% sodium thiosulfate; C. AMS 11.1 



Cajal 1913; D. MS 33.1 Gluck 1938; E. 2% formaldehyde; F. 0.2% gold chloride 

 method: [sections of F 3700.0000 Zenker 1894]^ water-* A, 4-8 hrs. -^^ 70% ale, 



wash — > water -^ B, 5 mins. -^ thorough wash -^ C, 24 hrs., 37°C. -^ wash -^ 7), 5 



mins. -^ rinse -^ E, 5 mins. — > wash — > F, till gray —^B,5 mins. — * wash — > balsam, 



via usual reagents 



33.41 Gridley 1951 Tech. Bull., 21:71 



reagents required: .4. 0.5% periodic acid; B. 2% silver nitrate; C. MS 33.1 da Fano 

 1919; D. 1.2% formaldehyde; E. 0.5% gold chloride; F. 5% sodium thiosulfate 



method: [6 n sections]—* water-* A, 15 mins. — > rinse -^ 7?, 30 mins. — » wash—* C, 

 15 mins. — * rinse — * 7), 3 mins. —>■ wash — » F, 5 mins. -^ wash -^ F, 5 mins. 



33.41 Gomori 1937 608b, 13 :993 



reagents required: A. 1% potassium permanganate; B.2% potassium metabisulfite; 



C. 2% ferric alum; D. MS 33.1 Gomori 1937; E. 4% formaldehyde; F. 0.2% gold 



chloride ; (?. 2 % sodium thiosulfate 

 method: [paraffin sections, brought to water, of formaldehyde-fixed material] —> A, 



1-2 mins. — * rinse —> B, I min. — > wash -^ C, 2 mins. — > wash — > 7), 1 min. — * quick 



rinse -* E, till reduced -^ wash -^ F, 10 mins. -^ rinse -^ B, 1 min. -^ wash ^ G, 1 



min. — > wash -^ balsam, via usual reagents 



33.41 Gordon and Sweets 1936a 608b, 12:545 



reagents required: A. 0.5% potassium permanganate 95, 3% sulfuric acid 5; B. 1% 

 oxalic acid; C. 2.5% ferric alum; D. MS 33.1 Gordon and Sweets 1936; E. 4% form- 

 aldehyde; 7'. 0.2% gold chloride; G. b% sodium thiosulfate 

 method: [sections, on slide, from fornuildehyde or F 3000.1010 material] -^ water -* A, 

 5 mins. — ► wash — * B, till white — * wash — * C, 15-20 mins. — * wash — * 7), few sees. -^ 

 rinse — > E, till reduction complete — > wash -^ F, 1-3 mins. — > wash — * G, 5 mins. —> 

 balsam, via usual reagents 

 recommended for: reticulum of spleen. 



