MS 33.41 METAL STAINS 593 



33.41 Gordon and Sweets 1936b 608b, 12:545 



ri<;agp:nts RKgriREi): .1. 0.5';;, |)()jassium prniKuifiaiiatc 05, .'}% sulfuric Mcid 5; /?. 1% 

 oxalic acid; C. 2.5% ferric alum; D. MS 'SAA Foot l'J27a; E. 4% formaldehyde; F. 

 0.2% gold chloride; G. 5% sodium thiosulfate 



method: [sections from formaldehyde or F 5000.1010 Bouin 1S97 material] -^ water —* 



A, 1-5 mins. -^ wash — > B, till white -^ wash — * C, 15 to 30 mins. —* wash — * D, few 

 sees. — > E, till brown — * wash — > F, l-,i mins. — > wash — + (r, 5 mins. — > wash — > bal- 

 sam, via usual reagents 



RECOMMENDED FORI reticulum of spleen. 



33.41 Jalowy 1937 23G.3nb, 27:6(57 



UEAGENTs REQUIRED: .4. MS 33.1 Jalowy 1937; B. 1% ammonia; ('. 10% neutralized 



formaldehyde 

 method: [sections of formaldehyde fixed materials] —>■ water — > A, 5-30 mins. 30°C. — » 



rinse — > B, rinse —^ C, 2-10 mins. — » balsam, via usual reagents 

 recommended for: collagen and reticular fibers in skin 



33.41 Krajian 1933 1789a, 16:376 



reagents required: A. 10% ammonia; B. 0.3% potassium permanganate; C. 1.5% 



oxalic acid; D. 5% silver nitrate; E. 33.1 Krajian 1933; F. 12% formaldehyde 

 method: [5-10 m sections by freezing technique of formaldehyde-fixed material] -^ water 



— > ^, 15 mins. 60°C. -^ wash —> B, 5 mins. -^ rinse -^ C, till decolorized — > wash — > 



D, 1 hr. 60°C. -^ j5;, 15 mins. 60°C. -^ wash ^ /^, 1-3 mins. 60°C. -* wash -» balsam, 



via aniline and xylene 

 recommended for: reticular and collagen fibers in frozen sections. 



33.41 Laidlaw 1929 608b, 5 :239 



REAGENTS REQUIRED: A. 1% iodine in 95% ale; B. 5% sodium thiosulfate; C. 0.5% 

 potassium permanganate; D. 5% oxalic acid; E. MS 33.1 Laidlaw 1929; F. 0.4% form- 

 aldehyde; G. 0.2% gold chloride 



method: [sections of F 5000.1010 Bouin 1897 fixed material] -^ water -^ A, 3 mins. -^ 

 rinse — » .B, 3 mins. -^ rinse — > C, 3 mins. — > wash — > D, 5 mins. — * wash — > E, 10 

 mins. —> wash -^ F, 10 mins. —> rinse —^ (7, 10 mins. wash —> balsam, via usual 

 reagents 



recommended for: reticulum fibers in skin. 



33.41 Levi 1907 14425, 18 :292 



REAGENTS REQUIRED: A. 2% silver nitrate; B. MS 33.1 Levi 1907; C. 5% formaldehyde; 



D. 0.5% gold chloride; E. 5% sodium thiosulfate 

 method: [paraffin sections of F 3700.0010 Zenker 1897 fixed material]—* water—* A, 



24 hrs. —* B, 20-40 mins., till brown — * rinse — > C, 5-10 mins. — * wash -^ D, 2 hrs. 



— » wash -^ E, 10-15 mins. -^ wash — > balsam, via usual reagents 

 recommended for: reticulum fibers in lymphatic glands. 



33.41 LUlie 1948 Lillie 1948, 187 



REAGENTS REQUIRED: A. 0.5% potassium permanganate; B. 5% oxalic acid; C. 1% 



uranium nitrate; D. MS 33.1 Lillie 1948; E. 4% formaldehyde; F. 0.2% gold chloride; 



G. 5% sodium thiosulfate 

 method: [paraffin in sections varnished on slide with colloidin] -^ A, 2 mins. — > wash — » 



B, 2 mins. — > wash —* C, 5-10 sees. — * D, on slide 3 mins. -^ rinse, E, 2 mins. -^ wash 

 — > F, 2 mins. — > wash — > G, 2 mins. — » wash — » balsam, via usual reagents 



33.41 Long 1948 see MS 33.44 Long 1948 



33.41 Maresch 1905 23681, 17:641 



REAGENTS REQUIRED: ^.2% silvcr nitrate; B. MS 33.1 Maresch 1905; C. 8% formalde- 

 hyde; D. 0.2% gold chloride in 0.2% acetic acid; E. 5% sodium thiosulfate 

 method: [sections of formaldehyde fixed material] — > A, 12-24 hrs. — > rinse—* B, 2-30 



mins. -^ wash -* C, 1 hr. — > D, 1 hr. -^ E, 30 mins. —>■ balsam, via carbol-xylene 

 recommended for: reticulum fibers in liver. 



