594 METHODS AND FORMULAS MS 33.41 



33.41 Negrin 1941 1887a, 31:108 



REAGENTS REQUIRED: A. 10% sodium Cyanide; B. MS 33.1 del Rio-Hortega 1921; C. 1% 



pyridine; D. 0.4% neutralized formaldehyde; E. 10% potassium thiocyanate; F. 1% 



gold chloride; G. 5% sodium thiosulfate 

 method: [sections] —» water —> a, 10-20 mins., 50°C. —» thorough wash -^ B, 10-20 



mins., 50°C. — » C, wash — > D, 1-3 mins. — » E, till differentiated —^ thorough wash 



—> F, 5 mins. — > wash — > G, 2 mins. -^ wash — > balsam, via usual reagents 



33.41 Perdrau 1921 11431,24:117 



REAGENTS REQUIRED: A. 0.25% potassium permanganate; B. DS 21.212 Weigert 1885; 



C. ADS 21.1 Pal 1887sols. A and 5; I>. 2% silver nitrate ;£•. MS33.1daFano 1919; F- 

 8% neutralized formaldehyde; G. 5% sodium thiosulfate 



method: [sections of formaldehyde fixed material] — > A, 10 mins. — > wash -^ B, 2 hrs. 

 -^ wash -^ C, {A) 15-20 sees. -^ C, {B) few sees. — > wash — + D, in dark, 12 hrs. -^ ^, 

 20-30 mins. -^ wash — » jP, 15 mins. -^ wash — » G, 5 mins. — * wash —> balsam, via 

 usual reagents 



33.41 del Rio-Hortega 1916a 21344, 14:181 



REAGENTS REQUIRED: A. 1% taunic acid in 95% alcohol; B. MS 33.1 del Rio-Hortega 



1916 10, water 90 (3 dishes required); C. 8% neutralized formaldehyde 

 method: [sections (see note below)] -^ A, 5 mins. at 55°C. — ♦ quick rinse —> B, 1st 



dish, 1 min. — ^ B, 2nd dish, 1 min. —* B, 3rd dish, 2 mins. -+ wash -^ C, 30 sees. -^ 



wash — > balsam, via usual reagents 

 note: del Rio-Hortega {loc. cit.) specifies formaldehyde material. Gatenby and Stern 



(Gatenby and Painter 1937, 549) state that alcohol or "Bouin" (presumably F 



5000.1010 Bouin 1897) material may be used provided that it be "re-formalized" 



for a few days; Cajal and de Castro 1933, 291, suggest that material from this fixative 



be stored in 4% formaldehyde. 



33.41 del Rio-Hortega 1916b 21344, 14:181 



reagents required: A. 1% tannic acid in 95% ale; B. MS 33.1 del Rio-Hortega 1916 



10, water 90 (3 dishes required); C. 1% gold chloride; D. 5% sodium thiosulfate 

 method: [sections by freezing technique of formaldehyde material] —> A, 5 mins., 55°C. 



— * rinse rapidly -^ B, 1st dish, 1 min. — > B, 2nd dish, 1 min. — > B, 3rd dish, till dark 



brown — * thorough wash — > C, 30 to 40 mins., 40°C. — > Z), 5 mins. -^ wash -^ balsam, 



via usual reagents 



33.41 Robb-Smith 1937 11431,45:312 



REAGENTS REQUIRED: A. 10% ammoiiia; B. 0.25% potassium permanganate; C. 1.5% 

 oxalic acid; Z). 5% silver nitrate ; .B. AMS33.1 Robb-Smith 1937;F. 15% formaldehyde; 

 G. 0.2% gold cUoride; H. 5% sodium thiosulfate 

 methods: [paraffin ribbons dried on slide, wax not removed] — > A, 15 mins. -^ wash 

 -^ B, 5 mins. — » wash -^ C, till bleached -^ wash -^ D, 1 hr. — ♦ rinse -^ E, 15 mins. 

 — > wash -^ F, Z mins. -^ wash G, 3 mins. -^ H, 1 min. — » wash —>■ dehydrate — > xy- 

 lene, till wax removed — » balsam 



33.41 Snessarew 1910 766,36:40 



REAGENTS REQUIRED: A. 5% ferric alum; B. 10% silver nitrate; C. MS 33.1 Bielschowsky 

 1902; D. 8% formaldehyde 



method: [sections by freezing technique of formaldehyde fixed material] — > A, 4 days 

 changed daily -* wash -^ B, 48 hrs. — > rinse — > C, 24 hrs. — > wash -^ D, till black- 

 ened -^ balsam, via usual reagents 



33.41 Studricka 1906 23632, 23:416 



REAGENTS REQUIRED: A. 3% silver nitrate; B. MS 33.1 Wolff 1905; C. 4% formaldehyde; 



D. 0.5% gold chloride; E. 5% sodium thiosulfate 



method: [sections of fixed and decalcified material] -^ A, 4 days — > wash -* 5, till 

 yellow-brown — > rinse — * C, till dark brown -* D, till gray-black -* wash —* E, 1 hr. 

 — > wash — ^ balsam, via usual reagents 



recommended for: reticulum fibers in cartilage, bone, and dentine. 



note: Zimmermann 1908 (23632, 25:8) differs only in reducing A to 2 days and in 

 diluting B fourfold. 



