MS 33.41-MS 33.42 METAL STAINS 595 



33.41 Urechia and Nagu 1931 6630, 106:498 



REAGENTS HioQi'iRED: .1. AMS 11.1 Cajal 1913; B. sat. sol. Miinnonimii sulfide; C. MS 



33.1 del Rio-llortega 1917a; D. 50% ale; E. 0.4% foniK.l.lciiy.l.'; /'. 0.2% gold 



chloride; G. 5% sodium thiosulfate 

 method: [fresh tissue]-^ .4, 3-4 days —> sections by freezing technique -► B, 15-30 



mins. -^ wash — > C, 2-4 hrs. — > D, quick wash, 2 changes-^ E, 5-10 niins. —^ F, 15 



mins. -^ wash — > G, 5 mins. -^ wash — * balsam, via usual reagents 

 RECOMMENDED kor: rcliculuiii fibers in nervous tissues. 



33.41 Wilder 1935a fi08b, 11:817 



RE.\GENT8 heqiired: ,4. 10% phosphouiolybdic acid; B. 1% uranium nitrate; C. MS 



33.1 Foot 1927a; D. AMS 21.1 Wilder 1935; E. 0.2% gold chloride; F. 5% sodium 



thiosulfate 

 method: [sections of F 3700.1000 or .1010 matcrial[ —> waters .4, 1 min. -^ wash -^ 



B, 5 sees. — » rinse — > C, 1 min. — > 95% ale. quick rinse -^ D, 1 min. — > wash — > E, 



1 min. -^ rinse -> F, 1-2 mins. -^ [dye stains as in MS 33.41 Foot 1924 to 1927b, if 



required] —* balsam, via usual reagents 

 RECOMMENDED FOR: reticuluui of spleen. 



33.41 Wilder 1935b 608b, 11:817 



REAGENTS REQUIRED: A. 1% potassium permanganate; B. 5% oxalic acid; C. 1% ura- 

 nium nitrate; D. Bielschowsky 1902 MS 33.1; E. AMS 21.1 Wilder 1935; F. 0.2% gold 

 chloride; G. 5% sodium thiosulfate 

 method: [sections] — > water — > ^, 1 min. —y rinse -^ B, 1 min. — + wash — > C, 5-10 sees. 

 -^ rinse D, 1 min. -^ 90% ale, rinse -^ ^, 1 min. -^ rinse -^ F, 1 min. — > rinse —> G, 

 1 min. -^ wash — > balsam, via usual reagents 

 recommended for: reticulum fibers. 



33.41 Zhookin 1937 11284,22:1284 



re.\gents required: .4. 0.001% potassium permanganate; B. MS 33.1 Lawrentjew 

 (1933); C. 0.04% formaldehyde, pH 7; D. 0.2% gold chloride; E. 5% sodium thio- 

 sulfate 

 method: [frozen sections of formaldehyde material] — > water ^ .4, 20 sees. -^ rinse — » 

 B, 2-3 mins. -^ rinse -+ C, till reduced -^ wash — > D, 1 min. —* E, 5 mins. -^ wash 

 — > balsam, via usual reagents 



33.41 Zimmermann 1908 see MS 33.41 Studnicka 1906 (note) 



33.42 SELECTIVE STAINING OF SPECIAL CELLS 



33.42 Gluckman 1943 4285a, 20 :63 

 REAGENTS REQUIRED: A. MS 33.1 Gluckmau 1943; B. 5% sodium thiosulfate 

 method: [parafhn sections of F 5000.1010 Bouin 1897 material] -^ water -^ A, \0 mins., 



60°C. —* B, 30 sees. — > wash — » balsam, via usual reagents 

 RECOMMENDED FOR: argcntiphil cells of intestine. 



33.42 Gomori 1946 519b, 10:177 



REAGENTS REQUIRED: A. 5% chrouiic acid ; B. 1% sodium bisulfite; C. MS 33.1 Gomori 



1946 50, water 50, sodium borate 0.2; D. 0.1 % gold chloride; .B. 2% sodium thiosulfate 

 method: [sections] -^ water —> ^, 1-1 J^^ hrs. —» wash -^ B, 1 min. -^ wash —> C, 1-3 



hrs. till glycogen granules dark brown -^ wash -^ D, 5 mins. —* E,b mins. — * wash — > 



[counterstain if desired] — * balsam, via usual reagents 

 recommended for: glycogen. 



33.42 Jacobson see MS 33.42 Masson 1923b (note) 



33.42 Masson 1923a test. 1937 Duthie Gatenby and Painter 1937, 414 



reagents required: A. 0.003% ammonia; B. MS 33.1 Fontanna 1912; C. AMS 22.1 



Cajal 1910; D. 2% sodium thiosulfate 

 method: [frozen sections formaldehyde fbced materials] -^^ .4, 2-3 hrs. -^ B, in dark, 



36 hrs. -^ wash — > C, 10 mins. — > wash — ♦ Z), 1 min. -^ wash — ► balsam, via usual 



reagents 

 recommended for: argentophil cells. 



