59G METHODS AND FORMULAS MS 33.42-MS 33.44 



33.42 Masson 1923b test. 1948 Lillie cit. Lee Lillie 1948, 102 



REAGENTS requiked: A. MS 33.1 Foatana 1912; B. 0.1% gold chloride; C. 5% sodium 



thiosulfate 

 method: [sections of formaldehyde material] -^ water -^ A, 12-48 hrs. in dark — >• wash 



— > B, 4-6 mins. — > wash — > C, 1 min. — > wash — > [counterstain if required] -^ balsam, 



via usual reagents 

 recommended for: argentaffin cells. 

 note: Lillie {loc. cit.) states that Lison (no ref.) "used the sequence given above" and 



that Jacobson (no ref.) omitted the gold chloride. 



33.42 Masson 1928 608b, 4:181 



REAGENTS REQUIRED: A. 0.1% ammonia; B. MS 33.1 Masson 1928; C. AMS 22.1 Cajal 



1948 

 method: [2-3 mm. slices of F 5000.1010 Bouin 1897 fixed material]-^ wash-* A, 24 



hrs. — > rinse — > B, 24 hrs. -^ rinse — » C, 24 hrs. — » [sections] 

 RECOMMENDED FOR: argentaffin cells. 



33.42 Ogata-Ogata 1923 2526, 71 :376 



REAGENTS REQUIRED: A. 1% ainmonia; B. MS 33.1 Maresch 1905 50, water 50; C. 3% 



sodium thiosulfate; D. 4% formaldehyde 

 method: [small blocks of fresh tissue] -^ A, 1-2 hrs. in dark -^ B, 3-5 hrs. in dark — > A, 



30 mins. changed several times, in dark -^ C, I hr. in dark — > wash — > D, 1-2 days —* 



[frozen sections] 

 RECOMMENDED FOR: chromaffin cells. 



33.42 Shanklin 1951 Cowdry 1952, 270 



REAGENTS REQUIRED: A. MS 31.1 Shanklin 1951; B. 5% sodium sulfite; C. 0.6%, pyri- 

 dine; D. MS 33.1 del RIo-Hortega 1927; E. AMS 21.1 Shanklin 1951; F. 0.2% gold 

 chloride; G. 5% sodium thiosulfate 



method: [7-10 m sections of formaldehyde material]—* water—* A, 24°C. 24 hrs.—* 

 wash -^ B, 1 hr. -^ C, 1-2 mins. -^ wash -^ D, 2-5 mins. -^ rinse -^ E, 1 min. -* 

 rinse -^ i^, 1 min. -* G, 1-2 mins. -* wash — > [counterstain if desired] — * balsam, via 

 usual reagents 



recommended for: parenchyma of pineal. 



33.43 DEMONSTRATION OF CALCIFIED AND OSSIFIED MATERIAL 



33.43 Gomori 1933 608b, 9 :253 



REAGENTS REQUIRED: A. 1.5% silvcr nitrate; B. water 100, sodium hypophosphite 5, 

 0.5% sodium hydroxide 0.2; C. 5% sodium thiosulfate; D. 7% sulfosalicylic acid 



method: [blocks of tissue or embryos not more than 2 mm. thick] -* 95% ale. 2-4 days 

 — * wash -^ A, 6-10 days, changed once or twice -^ thorough wash -* B, 4-8 days -^ 

 wash — * C, 2 days — > wash -^ D, till decalcified, 1-3 days -^ sections -^ [counterstain 

 if desired] 



recommended for: demonstration of calcium in tissues. 



33.43 Orban test. circ. 1938 Wellings Wellings circ. 1938, 190 

 REAGENTS REQUIRED: A. 0.25 %o potassium permanganate; B. 5% oxalic acid; C. 2% 



silver nitrate; D. MS 33.1 Bielschowsky 1902; E. 2% formaldehyde; F. 1% gold 



chloride; G. 5% sodium thiosulfate 

 method: [celloidin sections of decalcified, F 3700.1010 Heidenhain 1916 fixed, teeth] -* 



water -* A, 5 mins. -* wash -* B, 15 mins. —>■ wash -^ C, 24 hrs., in dark -^ wash -^ 



D, 30 mins. -^ wash -^ E, 30 mins. -^ wash -^ F,l hr., wash -> G, 2 mins. -♦ wash -» 



balsam, via usual reagents 

 recommended for: general structure of tooth pulp, and particularly development of 



dentine from it. 



33.44 OTHER HISTOLOGICAL METHODS 



33.44 Jacobson 1939 11431, 49:1 

 REAGENTS REQUIRED: A. MS 33.1 Foutaua 1912; B. 5% sodium thiosulfate 

 method: [sections of formaldehyde material] -^ water — * A, 12-24 hrs. -^ wash, 1 min. 



— > i?, 1 min. — > wash — * balsam, via usual reagents 

 recommended for: differentiation of argentophil cells in gastrointestinal tract. 



