MS 33.44-MS 33.51 METAL STAINS 597 



33.44 Long 1948 20540b, 23 :69 



REAGENTS REQUIRED! .1. 0.25% potassiuni permanganate; B. 5% oxalic acid; C. 10% 

 silver nitrate; D. MS 33.1 Long 1948; E. 0.05% ammonia; F. 0.4% neutralized form- 

 aldehyde;(r. 0.2% gold chloride; H. 5% sodium thiosulfate; /. 0.1%, azocarmine in 1% 

 acetic acid; J. 5% phosphotungstic acid; K. water 99, acetic acid 1, either light green 

 SFY (adult tissues) or fast green FCP' (embryonic tissues); L. 1% acetic acid 

 method: [sections of F 3700.1010 lieidenhain 1916 after removal of mercury with 

 iodine] -* water — > .A, 2 mins. -^ wash, 5 mins. -^ B, 2 mins. -> thorough wash -» C, 

 12 hrs., in dark -> wash -^ D, 25-30 mins., 40-45°C. -^ E, one dip -* F, 10-20 mins. 

 -^ wash -^ G, 15-30 mins. -^ rinse -^ H, 2 mins. -^ wash -^ I, 15-30 mins. -> rinse 

 -^ /, 3-6 hrs. —^ rinse -^ A', 3 mins. — > rinse -> L, rinse (or longer if differentiation 

 necessary) -^ dammar, via isopropyl alcohol and xylene 

 RECOMMENDED FOR: differentiation of reticular fibers (dense purple), collagen (green), 

 and myofibrils (red). 



33.44 Mclndoo 1928 1789a, 6:598 



REAGENTS REQUIRED: A. 10% formaldehyde; B. MS 33.1 del Rio-Hortega 1919 50, water 



50, pyridine 1; C. 20% formaldehyde buffered to pH 7; D. 2% sodium thiosulfate 

 method: [small pieces] -^ A, 20 days -^ [frozen sections] — > B, in watch glass heated to 



steaming, till brown -^ rinse -^ C, 1 min. -^ rinse -^ D, I min. -^ wash, 2-3 days — > 



balsam, via usual reagents 

 RECOMMENDED FOR: demonstration of bile capillaries. 



33.44 del Rio-Hortega 1926 3231, 26:107 



REAGENTS REQUIRED: A. MS 33.1 del Rio-Hortega (1933); B. 0.4% formaldehyde; C. 5% 



sodium thiosulfate 

 method: [frozen sections of formaldehyde material] —>■ water — » A, till brown, 50°-55°C. 



— » wash — > B, 5-10 mins. — » C, 5 mins. -^ wash — * balsam, via usual reagents 

 recommended for: epithelial fibrils. 



33.5 Bacteriological Methods 

 33.51 methods for spirochetes 



33.51 Ferguson test. 1916 Warthin 4349, 6:56 



reagents required: A. 2% silver nitrate; B. MS 33.1 Bielschowsky 1902; C. 8% form- 

 aldehyde; D. 0.01% gold chloride in 0.01% acetic acid; E. 5% sodium thicsulfate 



method: [sections] — > water -* A, 12-24 hrs. -^ B, 15-30 mins. -^ rinse — > C, 3 mins. — > 

 wash — > D, 2 mins. — > wash -^ E, 1 min. -^ balsam, via usual reagents 



33.51 Fontana 1912 7176,55:1003 



reagents required: A. F 0000.1010 Fontana 1912; B. AMS 13.1 Fontana 1912; C. MS 



33.1 Fontana 1912 

 method: [fresh smears] -> .4, 2 mins. -^• wash -^ B,5 sees., 40°C. -^ wash -♦ C, dropped 



on slide and warmed, 2-30 sees. — > repeat, until sufficiently darkened — > wash — > dry 



— > balsam, if permanent preparation is required 

 note: Langeron 1942, 636 recommends F 0000.1010 Ruge 1942 in place of A above. 



33.51 Gordon 1936 see 33.6 Gordon 1936 



33.51 Lancelin, Seguy, and Debreuil 1926 6630, 94:557 



reagents required: A. F 0000.1010 Fontana 1912; B. AMS 13.1 Fontana 1912; C. MS 



33.1 Fontana 1912; D. AMS 22.1 Lancelin, Seguy, Debreuil 1926 

 method: [fresh smears] -* A,2 mins. -^ wash -^ B,5 sees., 40°C. -^ wash — » C, dropped 



on slide and warmed, 20-30 sees. -^ rinse -^ D, till sufficiently darkened -^ dry -^ 



balsam, if permanent preparation required 



33.51 Krajian 1933 test. 1935 abstract 20540b, 10:1935 



REAGENTS REQUIRED: A. 2% sodium cobaltinitrite; B. AMS 12.1 Krajian 1933; C. MS 



33.1 Krajian 1933; D. AMS 21.1 Krajian 1933 

 method: [5 M frozen sections of formaldehyde material]-^ A, 5 mins. -^ wash -^ B, 



15 mins. -> rinse — > C, 15-25 sees. — > wash — > D, 5 mins. — ♦ wash -^ balsam, via 



usual reagents 

 note: See also Krajian MS 31.51 Krajian 1935. 



