598 METHODS AND FORMULAS MS 33.51-MS 33.6 



33.51 Langeron 1942 see MS 33.51 Fontana 1912 (note) 



33.51 Seguin 1939 829, 10:838 



REAGENTS REQUIRED: A. AMS 21.1 van Ermengen 1940; B. 33.1 Fontana 1912; C. 0.5% 



gold chloride; D. 0.25% platinic chloride 

 method: [osmic fixed smears] -^ dry — > abs. ale. 10 mins. — > dry -^ A, on slide, 10 mins. 



-^ thorough wash — > B, 10 mins. — > wash — > C, few mins. — > rinse -^ D, few mins. — » 



dry 



33.51 Steiner 1937 11284,23:293 

 REAGENTS REQUIRED: A. 1.5% ammonia; B. MS 33.1 Steiner 1937 



method: [air-dried films] -^ A, till dehemoglobinized -^ wash — * 5, on slide, 40-90 mins. 

 -^ drain —> wash — > dry 



33.52 OTHER BACTERIOLOGICAL METHODS 



33.52 Craigie 1928 3566,9:55 



reagents required: A. AMS 13.1 Zettnow 1891; B. MS 33.1 Craigie 1928; C. 0.03% 



gold chloride 

 method: [smears from formolized suspensions] — > dry, 37°C. -^ heat, 100°C., 5 mins. -^ 



water, 5 mins. -^ rinse -> dry, 100°C., 5 mins. — > A, 5-10 mins., 100°C. -^ wash -^ fi, 



on slide, warmed to steaming and held warm till smear brown -^ wash —>■ C, 30 mins. 



— » wash -^ dry 

 recommended for: bacterial smears. 



33.52 Novel 1939 857, 63:302 



reagents required: A. water 100, tannic acid 10, ferrous sulfate 16, magenta 0.4; 



B. MS 33.1 Fontana 1912 

 method: [air dry smears] — > A, 1-1,^2 niin. -^ rinse -^ B, till chocolate, }i to 13^^ min. 



— > rinse — * dry 

 recommended for: bacterial fiagella. 



33.52 Zettnow 1891 23684, 11:689 



REAGENTS REQUIRED: A. AMS 13.1 Zettuow 1891; B. MS 33.1 Zettnow 1891 



method : [Smear preparations on coverslip] — > A, in dish heated on water bath, 5-7 mins. 



— > water, wash -^ B, poured on slip, heated to steaming till margin blackens — > wash 



-^ dry 

 recommended for: bacterial fiagella. 



33.6 Other Silver Diammine Methods 



33.6 Gordon 1936 11284, 22:294 



REAGENTS REQUIRED: A. 2.5% ferric alum; B. water 100, gelatin 1, 2% sodium car- 

 bonate 0.1; C. MS 33.1 Gordon 1936; Z>. AMS 21.1 Gordon 19.36 

 method: [formaldehyde fixed smears] -^ A, 10 mins. -^ wash -^ B, dip, drain —>■ quick 

 rinse -^ C, 5-15 mins. -^ wash at 60°C. -^ D, till reduced -* wash -^ balsam, via 

 usual reagents 

 recommended for: blood smears containing parasites. 



33.6 Haymaker and Sanchez-Perez 1935 19938, 82 :355 



REAGENTS REQUIRED: A. watcr 90, 40% neutralized formaldehyde 10, sodium chloride 

 0.45; B. 0.5%, ammonia; C. MS 33.1 Haymaker and Sanchez-Perez 1935; D. MS 33.1 

 del Rio-Hortega 1923 50, water 50; E. 0.4% formaldehyde; F. 0.2% gold chloride; G. 

 1% sodium thiosulfate 

 method: [clots on coverslip] -* A, 24 hrs. -+ B, 5 mins. -^ wash -> C, 40°C. till clot 

 yellows (about 10 mins.) -^ wash -^ D, 40°C. till clot brown (about 9 mins.) -♦ wash 

 -->■ E,5 mins. -» F, 40°C. till violet (about 10 mins.) -^ G, 5 mins. -^ wash -^ balsam, 

 via usual reagents 

 RECOMMENDED FOR: cells in tissue culture. 



33.6 Kalwaryjski 1938 test. 1939 Findlay 11360, 59:36 



REAGENTS REQUIRED: A. ADS 12.2 Lugol ; B. 2.5% sodium thiosulfate; C. MS 33.1 Kal- 

 waryjski 1938; D. 5% sodium thiosulfate 



