G04 METHODS AND FORMULAS MS 34.21 



method: [large pieces] -^ A,2 wks. or until firm -^ [3 mm. slices from A]-^ B,3 days — > 

 drain or blot -^ 25 ml. C, agitate gently -^ repeat successive washings in 25 ml. C, till 

 no further ppt. appears in solution — »• D, 2-3 days at 26°C. -^90% alcohol, rinse — > 

 [frozen, celloidin, or freehand sections] -^ dammar or cedar oil ivilhout cover 



34.21 Bolton 1898 see MS 34.21 Gerota 1896 (note) 



34.21 Brookover 1910 see MS 34.21 Golgi 1880 (note) 



34.21 Bubenaite 1929 23632, 46 :359 



REAGENTS REQUIRED: A. 2.5% potassium dichromate; 5. 2% silver nitrate 



method: [pieces of formaldehyde-fixed material]-^ A, 2 days, 34°C. -^ B, rinse —> B, 



fresh solution, 1-2 days, 34°C. -^ wash -^ [paraffin sections] 

 RECOMMENDED FOR: ganglion cells. 



34.21 Cajal 1890 see MS 34.21 Golgi 1880 (note) 



34.21 Cajal 1891 Cajal' s double impregnation — auct. 6011,8:130 



REAGENTS REQUIRED: A. F 1700.000 Golgi 1880; B. 0.75% silver nitrate; C. F 1700.0000 

 Cajal 1891 



method: [small blocks, fresh tissue] -^ 100 ml. A, 2-3 days -^ drain -^ 25 ml. B, agitate 

 gently -^ repeat successive washings in 25 ml. B, until no further ppt. appears in solu- 

 tion -^ 100 ml. B, 24 to 48 hrs. -^ rinse -* A or C, 1-2 days -^ drain -^ wash in B as 

 above -^ 100 ml. B, 36-48 hrs. ^^ 90% ale. quick wash -^ sections, either frozen, free- 

 hand or collodion -^ dammar or cedar oil without cover 



note: Durig 1895 (766, 10:659) substitutes Durig 1895 F 7000.1000 for A and C above. 



34.21 Durig 1895 see MS 34.21 Cajal 1891 (note) 



34.21 Fish 1895 see MS 34.21 Golgi 1880 (note) 



34.21 Gerota 1896 10157, 13:108 



reagents required: A. 4% neutralized formaldehyde; B. 4% potassium dichromate; 



C. 0.5% silver nitrate 

 method : [whole brains] -^ A, very large volumes, 1-2 wks. — * [small pieces from A] —^ B, 

 3-5 days — > drain, or blot -^ 25 ml. C, agitate gently -^ repeat successive washings to 

 25 ml. C, until no further ppt. appears in solution -^ 100 ml. C, 10-20 days -^95% 

 alcohol quick wash — » [sections, either freehand, frozen or collodion] — * dammar, or 

 cedar oil, without cover 

 note: Bolton 1898 (11360, 20:244) differs from above in substituting 1% ammonium 

 dichromate for B, and 1 % silver nitrate for C. 



34.21 Golgi 1875 test. 1903 ips. Gohji's slow process — auct. 



Golgi 1903, 1:128 



reagents required: A. F 7000.0000 MuUer 1859; B. potassium dichromate; C. 0.75% 

 silver nitrate 



method: [small blocks, fresh tissue] — > 100 ml. A, 5 days — > add 1 gm. B, leave 5 days — > 

 add 1 Gm. B, leave 5 days — > add 1 Gm. B, leave until sufficiently hardened — ♦ drain 

 or blot — > 25 ml. C, agitate gently -^ repeat successive washings until no further ppt. 

 appears in solution — ♦ 100 ml. C, 24 to 48 hrs. — > 90% ale, changed when discolored, 

 till required -^ [sections, either frozen, freehand, or collodion] -^ dammar, or cedar oil 

 without cover 



note: a detailed description of this technique is given under MS 34.0 above. 



34.21 Golgi 1880 test. 1903 ips. Golgi' s quick process — auct. 



Golgi 1903, 1:162 

 reagents required: A. F 1700.0000 Golgi 1880; B. 0.75% silver nitrate 

 method: [small blocks, fresh tissue] -^ 100 ml. A, 2 to 3 days -^ drain -^ 25 ml. B, agi- 

 tate gently — > repeat successive washings in 25 ml. B until no further ppt. appears in 

 solution -^ 100 ml. B, 24 to 48 hrs. or until required -^90% ale. quick wash — + [sec- 

 tions, either frozen, freehand, or collodion] —* dammar or cedar oil without cover 

 note: Cajal 1890 substitutes F 1700.0000 Cajal 1890 for A above. Lachi 1895 (14425, 

 5:15) substitutes F 7000.1000 Lachi 1895 for 48 hours for A above, dell' Isola 1895 



