MS 34.21 METAL STAINS 605 



(3248, 2) substitutes F 1700.1000 dell' Isola 1895 for A above. Strong 1895 (266, 

 10:494) substitutes F 7000.1000 Strong 1895 for A above. Fish 1895 (21400a, 17:319) 

 substitutes either F 1700.1000 Fish 1895 or F 7000.1000 Fish 1895 for A above using 

 3 days in both A and B. Brookover 1910 (11135, 20 :49) precedes A by fixation in 4% 

 neutralized formaldehyde. Vassale and Donaggio 1895 (14425, 6:82) substitute their 

 F 7000.2000 for A above. Andreizen 1894 (3579, 1:909) substitutes his method 

 (F 1700.0000) of fixation for A above. Hill 1896 (34G4, 9:1) substitutes MS 34.1 Hill 

 1896 for B above. Sehrwald 1889 (23632, 6:456) and Mann 1902 {test, da Fano 1936 

 in Gatenby and Painter 1937, 509) coat the object with 10% gelatin before trans- 

 ferring to silver, 



34.21 Golgi 1900 test. 1903 ips. Golgi's mixed process — auct. 



Golgi 1903, 2 :685 

 REAGENTS REQUIRED: A. 2% potassium dichromate; B. F 1700.0000 Golgi 1900; C. 



0.75% silver nitrate 

 method: [small blocks of fresh tissue] — > yl, 2 to 30 days -^ 5, 3 to 19 days — » drain or 

 blot -^ 25 ml. C, agitate gently — > repeat successive washings in 25 ml. C until no 

 further ppt. appears in solution —>■ 100 ml. C, 24 to 48 hrs. or until required ^90% 

 ale, quick wash -^ [sections, either frozen, freehand, or collodion] — > dammar, or 

 cedar oil, without cover 



34.21 Golgi test. 1933 Cajal and de Castro Cajal and de Castro 1933, 357 



REAGENTS REQUIRED: A. F 8000.1000 Lawrcntjcw (1933); B. 2% silver nitrate; C. AMS 



21.1 Cajal 1914 

 method: [pieces of tissue] — > A, 8-10 days — > B, rinse — > B, fresh sol., 5-7 days -^ wash 



-^ C, 24 hrs. -^ sections 

 RECOMMENDED FOR: nervc endings. 



34.21 Hill 1896 see MS 34.21 Golgi 1880 (note) 



34.21 deir Isola 1895 see MS 34.21 Golgi 1880 (note) 



34.21 Juschtschenko test. 1933 Cajal and de Castro Cajal and de Castro 1933, 124 



REAGENTS REQUIRED: A. F 1700.0000 Cajal 1890; B. MS 34.1 Juschtschenko (1933) 

 method: [sympathetic ganglia] — > A, 1-5 days — + quick wash —^ blot dry — * B, 2-3 days 



-^ [section] — > dammar, or cedar oil, without cover 

 RECOMMENDED FOR: Sympathetic ganglia. 



34.21 Kolossow 1897 test, da Fano Gatenby and Cowdry 1928, 608 



REAGENTS REQUIRED: A. F 1700.0000 Kolossow 1897; B. MS 34.1 Kolossow 1897 

 method: [whole ganglia] —> A, 1-7 days -^ rinse and blot -^ B, 2-3 days -^ 90% ale, 



quick wash —y [sections either frozen, freehand, or collodion] — > dammar or cedar oil 



without cover 

 recommended for: sympathetic ganglia. 

 note: a detailed description of this technique is given under MS 34.0 above. 



34.21 Kopsch 1896 766, 11 :727 



REAGENTS REQUIRED: A. F 7000.1000 Kopsch 1896; B. 3.5% potassium dichromate; C. 



0.75% silver nitrate 

 method: [small pieces, fresh tissue] — > 100 ml. A, 24 hrs. in dark —>■ 100 ml. B, 48 hrs. —y 

 drain -^ 25 ml. C, agitate gently — > repeat successive washings in 25 ml. C until no 

 further ppt. appears in solution — ♦ 100 ml. C, 2 to 6 days ^95% ale, quick wash — ► 

 [sections, either freehand, frozen, or collodion] -^ dammar, or cedar oil, without cover 

 note: Schreiber 1898 substitutes his F 7000.1000 for A above and his F 7000.1000 for B 

 above. 



34.21 Lachi 1895 see MS 34.21 Golgi 1880 (note) 



34.21 Lawrentjew test. 1933 Cajal and de Castro Cajal and de Castro 1933, 359 



REAGENTS required: A. F 8000.1000 Lawrentjew 1933; B. S% neutralized formalde- 

 hyde; C. 20% silver nitrate; D. MS 33.1 Lawrentjew 1933; E. 30% ammonia; F. 0.3% 

 gold chloride; G. 5% sodium thiosulfate 



