606 METHODS AND FORMULAS MS 34.21-MS 34.22 



method: [fresh pieces] ^ ^, 1 hr. — > B, 4-6 days — * wash -^ [frozen sections] -^ C, 1-5 

 mins. -^ B, 1-2 mins. -^ D, till nerve endings clearly shown —* E, I min. —^ wash —^ 

 F, till gray — >• G, 5 mins. -^ balsam, via usual reagents 



RECOMMENDED FOB: peripheral nerve endings. 



34.21 Mann 1902 see MS 34.21 Golgi 1880 (note) 



34.21 Sala 1891 23632, 9 :389 



REAGENTS required: .4. F 1700.0000 Golgi 1880; B. 0.75% silver nitrate 

 method: [entire ganglion] -^^ .1, 3 days — > rinse — * B, 2-3 days — * rinse — > A, 4 days — > 

 wash — > sections by freezing technique — » dammar or cedar oil without cover 



34.21 Sanchez test. 1933 Cajal and Castro Cajal and Castro 1933, 128 



REAGENTS REQUIRED: A. F 7000.1000 Sdnchez 1933; B. 0.5% silver nitrate; C. 0.75% 



silver nitrate 

 method: [invertebrates] — » A, changed daily, 3-5 days — > B, with agitation, 1-2 mins. 

 -^ C, 2-3 days—* A, with agitation, 1-2 mins. — > A, changed daily, 1-3 days ^ B, 

 with agitation, 1-2 mins. — > C, 1-3 days -^ [celloidin sections] 

 recommended for: nerve fibers in invertebrates. 



34.21 Schreiber 1898 see MS 34.21 Kopsch 1896 (note) 



34.21 Sehrwald 1889 see MS 34.21 Golgi 1880 (note) 



34.21 Smith 1930 Turtox News, 8:91 



RE.AGENTS REQUIRED: A. 4% potassium dichromate; B. 0.75% silver nitrate 



method: [2 mm. slices of formaldehyde material]-^ rinse—* A, 3-5 days — > wash — > 



blot — * B, 15 mins. with constant agitation —^ B, fresh solution, 24 hrs. — > section 

 recommended for: general neurology. 



34.21 Smirnow 1895 1780, 52:201 



REAGENTS REQUIRED: A. F 7000.1000 Smirnow 1895; B. 3.5% potassium dichromate; C. 



F 1700.1000 Smirnow 1895; D. 0.5% silver nitrate; E. 1.0% silver nitrate 

 method: [fresh whole cerebellum] -^ A, 1-8 wks. — > [split halves from A] —* B, 2-5 wks. 



-^ [1 cm. slices from B] — > C, 7 to 10 days — > drain or blot -^ 100 ml. D, 24 hrs. -^ E, 



36-48 hrs. -^ [frozen, celloidin, or freehand sections] -^ dammar, or cedar oil, without 



cover 



34.21 Strong 1895 see MS 34.21 Golgi 1880 (note) 



34.21 Timofecheff test. 1933 Cajal and de Castro Cajal and de Castro 1933, 125 



REAGENTS REQUIRED: A. F 1700.0000 Tiiiiofecheff 1933; B. water 100, silver nitrate 1, 



formic acid 0.01, sodium sulfate 0.001 

 method: [pieces of fresh tissue of 1 cm. side] -^ A, 6-7 days -^ quick wash blot — > B, 2-3 



days -^ [section] 

 recommended for: nerve endings in male sex organs. 



34.21 Vassale and Donaggio 1895 see MS 34.21 Golgi 1880 (note) 



34.21 Windle 1926 11135,40:229 



REAGENTS REQUIRED: A. 3.5% potassium dichromate; B. F 1700.0000 Windle 1926; 



C. 0.75% silver nitrate 

 method: [fetal brains] —> A, 48 hrs. — * B, 72 hrs. -^ .4. 5-6 days — > drain or blot — > 



25 ml. C, agitate gently —^ repeat successive washings in 25 ml. C, till no more ppt. 



produced in solution — ► 100 ml. C, 7-10 days in dark — » [half brains from C]-* 95% 



alcohol, till dehydrated —>■ [sections by celloidin technique] -> dammar, or cedar oil 



without cover 

 note: a detailed description of this technique is given under MS 34.0 above. 



34.22 NEUROGLIA 



34.22 Cajal test. 1933 ips. Cajal and de Castro 1933, 282 

 REAGENTS REQUIRED: A. 6% formaldehyde; B. F 7000.1000 Cajal 1933; C. 1% silver 



nitrate; D. 1% silver nitrate in 1% chloral hydrate 



