MS 34.22-MS 34.32 METAL STAINS G07 



method: [small pieces of fresh tissue]—* A, 2-5 days — > B, 3-5 days — > C, with rapid 

 agitation -^ C, fresh sol. — > repeat till clouds of ppt. cease — > Z), 2 days -^90% ale. 

 wash —^ [paraffin sections] -> dammar or cedar oil ivithoul cover 



RECOMMENDED FOR: oligodendria. 



34.22 Martinez 1931 3232, 31 :653 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1913; B. 0.5% ammonia; C. MS 34.1 Martinez 



1931; D. 4% formaldehyde; E. 0.2% gold chloride; F. 5% sodium thiosulfate 

 method: [fresh pieces] —> .1, 1 hr., 55°C. -^ wash ^ [frozen sections] —> B, 5 mins. -^ 

 rinse —>■ C, 15 mins. -^ wash -^ D, till pale yellow -^ wash — + E, 5 mins. — > E (fresh 

 solution), 15 mins., 50°C. — > F, till purple -^ wash — > balsam, via usual reagents 



34.22 del Rio-Hortega 1928 list. 1933 Cajal and de Castro 



Cajal and de Castro 1933, 281 

 reagents required: A. F 7000. lOOO del Rio-Hortega 1928; B. 1.5% silver nitrate 

 method: [fresh blocks of tissue] — > .4, renewed daily, 2-3 days —^ rinse —* B, 2ot 3 days 



— > 90% ale, wash —> [paraffin sections] — » dammar or cedar oil without cover 

 recommended for: oligodendria. 



34.3 Histological Methods 



34.31 reticulum fibers 



34.31 Amprino 1936 4285a, 13 :223 



reagents required: .1. 2% tannin in 95% ale; B. MS 34.1 Amprino 1936 30, water 



100; C. 15% formaldehyde 

 method: [sections of formaldehyde-fixed material] —♦ water —+ A, 30-40 mins., 55- 

 58°C. -^ water — > B, few mins. — > B (fresh solution), few mins. — > B (fresh solution), 

 till light yellow — > rinse -^ C, till reduced — > balsam, via usual reagents 



34,31 Oliveira 1936 22575, 298 :523 



reagents required: A. 10% phosphomolybdic acid; B. 1% uranium nitrate; C. 



MS 33.1 Foot 1924; D. AMS 21.1 Oliveira 1936a; E. MS 34.1 Oliveira 1936; F. AMS 



21.1 Oliveira 1936b; G. 0.2% gold chloride; H. 5% sodium thiosulfate 

 method: [paraffin sections] -^ A, I min. — » rinse —fB,5 sees. — + quick rinse -^ C, 1 min, 



-^95% ale, rinse — > D, 1 min. -^ wash -^ E, 15-20 mins. 56°C. — > wash -^ F, \ min. 



-^ wash—* G, 5-10 mins. 56°C. -^ H, 6-10 mins. —> thorough wash — > balsam, via 



usual reagents 



34.31 Oppel 1890 766, 4:144 

 reagents required: A. 5% potassium chromate; B. 0.75% silver nitrate 

 method: [small pieces ale. fixed material] — > water — » A, 24 hrs. —>■ wash -^ B, 24 hrs. — > 



wash — > [frozen tangential sections] 

 recommended for: lattice fibrils in liver. 



34.32 other histological methods 



34.32 Kupffer 1889 20188, 5:219 

 REAGENTS REQUIRED: A. F 1700.0000 Cajal 1890; B. 0.75% silver nitrate 

 method: [small pieces of liver] -^ A, 24 hrs. —> wash —>■ B, 48 hrs. — > [sections] 



34.32 Martinotti 1888 test. 1889 Behrens, Kossel, and Schiefferdecker 



Behrens, Kossel, and Schieffer- 

 decker 1889, 211 

 REAGENTS REQUIRED: A. 2% arseuic trioxide; B. F 7000.0000 Mtiller 1859; C. water 12, 



silver nitrate 8, glycerol 80; D. 0.75 sodium chloride 

 method: [small pieces] -^ A, till decalcified -^ wash — » B, 15 mins. -^ rinse —>■ C, 24-48 

 hrs. — > rinse — > sections — » 95% ale. — + D, few minutes -^ balsam, via usual reagents 

 recommended for: elastic fibers, 



34.32 Regaud and Dubreuil 1903 trst. 1907 Bohm and Oppel 



Bohm and Oppel 1907, 175 

 reagents required: A. 1% protein silver 50, 1% osmic acid 50; B. any DS 11.2 stain 

 method: [fix flat sheet of epithelium in A]—> wash — > B, till nuclei are stained 

 recommended for: delineation of cell outlines in epithelium. 



