^^^ METHODS AND FORMULAS MS 34.32-MS 35.1 



34.32 Renaut test. 1907 Bohm and Oppel cit. Regaud Bohm and Oppel 1907, 387 



REAGENTS REQUIRED: A. MS 34.1 Renaut (1907) 



method: " a " is copiously injected into the testes, which are then hardened in alcohol. 

 Frozen sections are then mounted in balsam and exposed to light. 



RECOMMENDED FOR: demonstration of lymph vessels in testes. 

 34.32 Schultze 1907 1780, 69:544 



REAGENTS REQUIRED: A. Water 100, sUver nitrate 2, osmic acid 0.1; B. 1% hydroquinone 



method: [pieces of tissue] -> A, 30 mins. -^ rinse -» B, 24 hrs. -^ wash -* balsam, via 

 usual reagents 



recommended for: demonstrating outlines of epithelial cells. 



34.4 Cytological Methods 

 34.4 Aoyama 1930 23632, 46:490 



REAGENTS REQUIRED: A. F 8000.1000 Aoyama 1930; B. 1.5% silver nitrate; C. AMS 21.1 



Aoyama 1930 

 method: [small pieces] ^ A, 3-4 hrs. -^ rinse -^ B, 10-15 hrs., 25°C. -^ rinse -> C, 5-10 



hrs. -^ [paraffin sections] 

 RECOMMENDED FOR: Golgi network. 



34.4 Bubenaite 1937 test. 1937 Gatenby and Painter Gatenby and Painter 1937, 313 

 REAGENTS REQUIRED: A. 4% formaldehyde; B. 25% potassium dichromate; C. 2% 



silver nitrate 

 method: a, 1-2 days -» rinse -> 5, 2 days 35°C. -^ rinse -> C, wash, renewing solution 



when necessary, till no further ppt. occurs in solution -^ C, fresh solution, 1 to 2 days 



35°C. -^ [sections] 

 RECOMMENDED FOR: Golgi apparatus. 



34.4 Golgi-Verratti test. Cajal and de Castro Cajal and de Castro 1933, 200 



REAGENTS REQUIRED: A. F 1270.0000 Veratti 1890; B. water 100, potassium dichromate 



2, copper sulfate 1; C. 1% silver nitrate 

 method: [fragments of fresh tissue]^ A, 3-4 wks. ^ wash^ B, overnight-^ C, 4-8 



hrs. — > [sections] 

 RECOMMENDED FOR: Golgi apparatus. 



34.4 Veratti see MS 34.4 Golgi- Veratti 1933 



34.5 Bacteriological IVIethods 



34.5 Fontana 1923 9170, 64:234 



REAGENTS REQUIRED: A. F 0000.1010 Rugc 1942; B. 0.75% neoarsphenamine or AMS 

 13.1 Iron 1924; C. Fontana 1912 MS 33.1 



method: [fresh smears] -^ A,2 mins. -» wash -^ B, dropped on slide and warmed, 20-30 

 sees. -^ wash -^ C, dropped on slide and warmed, 20-30 sees. -^• repeat until suffi- 

 ciently darkened -^ wash -^ dry — > balsam 



recommended for: spirochetes in smears. 



34.5 Safford and Fleisher 1931 20540b, 6:43 



REAGENTS REQUIRED: A. AMS 13.1 Safford and Fleisher 1931; B. MS 33.1 Fontana 1912 

 method: [moist smears] -^ A, heated to steaming, 2 mins. -^ wash -^ dry -^ B, heated 



to steaming, 1-2 mins. -^ wash -^ dry 

 recommended for: bacterial flagella. 



34.5 Williams test. 1924 Mallory and Wright Mallory and Wright 1924, 279 



note: This method involves a proprietary developer of undisclosed formula and cannot 

 be further noticed. 



35 OTHER SILVER METHODS 



35.1 Staining Solutions 



35.1 David 1934 23684,132:240 



preparation: Dissolve 1 silver nitrate in 4 water. Add to 1.2 sodium sulfate in 2 hot 

 water. Wash ppt. by decantation and suspend in 100 water. 



