MS 35.1-MS 41.2 METAL STAINS 609 



35.1 Lugaro 1932 tesl. 1933 Cajal and de Castro Cajal and de Castro 1933, 260 



Stock I. 9% sodium thiosulfate; Stock II. 2% silver bromide in 9% sodium thiosulfate; 

 Stock III. 0.3% silver iodide in 9% sodium thiosulfate 



II III 



Working solutions 



G 10 10 8 



H 11 9 12 



35.2 Neurological Methods 



35.2 Lugaro 1932 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 260 

 REAGENTS REQUIRED: .4. MS 35.1 Lugaro 1932; B. 8% formaldehyde 



method: [pieces fixed 3-8 days in 7% formaldehyde on ice] — ♦ 10 ml. A in flask in dark, 

 3 hrs. -^ 7 ml. B added to flask, 5-8 days -^ wash -^ frozen sections -^ balsam, via 

 usual reagents 



recommended for: neuroglia. 



note: Any one of the eight working solutions given under 33.1 Lugaro 1932, or an as- 

 sortment of them, may be selected. 



35.3 Other Methods 



35.3 David 1934 23684, 132 :240 

 REAGENTS REQUIRED: A. ADS 12.2 David 1934; B. MS 35.1 David 1934 



method: [heat-fixed smear] -^ A, IQ mins. -^ wash — » heat dry -^ B, heated to steam- 

 ing, till brown — > wash — > dry 

 recommended for: bacterial flagella. 



MS 40 Other Metals 



41.1 Staining Solutions 



41.1 del Rio-Hortega 1927 test. 1933 Cajal and de Castro 



Cajal and de Castro 1933, 279 

 formula: water 94.5, hydrochloric acid 5.5, potassium ferrocyanide 2.25 



41.2 Neurological Methods 



41.2 Azoulay 1894 4956, 49 :924 

 REAGENTS REQUIRED: A. 0.5% ammonium vanadate; B. 2.5% tannin 



method: [thin celloidin sections of dichromate fixed material] — > A, poured over section 

 on slide, 2-30 sees. — » rinse -> B, poured over section on slide, 20-30 sees. — > [repeat 

 A — > rinse — ♦ B, till staining sufficient] -^ balsam, via usual reagents 



recommended for: cerebellum. 



41.2 Cox test. 1933 Cajal and de Castro Cajal and de Castro 1933, 130 



REAGENTS REQUIRED: A. F 3700.0000 Cox 1895; B. 5% sodium bisulfite 

 method: [large pieces] — » A, 2-3 months -^ 90% ale, till mercuric chloride extracted — > 



[celloidin sections] -^ B, till dark brown -^ M 23.1 Cox 1891 

 . recommended for: general neurology. 



41.2 Flater 1895 1780,45:158 



REAGENTS REQUIRED: A. 4% potassium dichromate; B. 0.1% mercuric chloride 

 method: [whole brains]-^ A, 2-3 months-^ 0.5 mm. slices-^ B, changed when dis- 

 colored, 9-12 months — » wash — > [celloidin sections] -^ balsam, via carbol-xylene 

 recommended for: brains of small vertebrates. 



