610 METHODS AND FORMULAS MS 41.2-MS 41.3 



41.2 Golgi 1878 Golgi 1903, 1:143 



REAGENTS REQUIRED: A. 1% potassium dichromate; B. 1.5% potassium dichromate ; C. 



2% potassium dichromate; D. 1% mercuric chloride 

 method: [large pieces] -^ ^, 1 month —> B, 1 month — * C, 1 month or till required —* 

 1-2 cm. cube blocks^ D, changed daily, till block is decolorized-^ wash—* [cel- 

 loidin sections] -^ wash -> AMS 24.1 Golgi (1937) -^ M 23.1 mountant 

 recommended for: axis cylinder and dendrites in brain. 



41.2 Krohntal 1899 23632, 16 :235 



REAGENTS REQUIRED: A. F 8000.1000 Krohntal 1899; B. water 90, 40% formaldehyde 10, 



hydrogen disulfide to sat. 

 method: [fresh tissue]—* A, 4-5 days — > B, with agitation, few mins. — * B, changed 



daily, 5 days —* [celloidin sections] 

 recommended for: general neurology. 



41.2 Pollaillon test. circ. 1938 Wellings Wellings circ. 1938, 132 



reagents required: A. 10% ferric chloride; B. 30% tannic acid 

 method: [sections to water]—* A, 24 hrs. -^ wash — > B, till dark enough ^^ wash — > 



M 11.1 mountant 

 recommended for: nerve fibers in teeth. 



41.2 vom Rath 1895 see F 1250.0010 vom Rath 1895 



41.2 del Rio-Hortega 1927 test. 1933 Cajal and de Castro 



Cajal and de Castro 1933, 278 



reagents required: A. AMS 11.1 Cajal 1913; B. 0.1% ammonia; C. 10% hydrochloric 

 acid; D. MS 41.1 del Rio-Hortega 1927; E. 1% sodium carbonate; F. 0.1% hydro- 

 chloric acid 



method: [blocks of fresh tissue]-^ A, some days -^ [20-25 m frozen sections]—* B, 

 thorough wash -^ thorough wash — * C, 1 min. — > D, 50°-60°C., with gentle rocking, 

 until solution begins to cloud -* E, until sections transparent — * F, few sees. — > [re- 

 peat D —* E —^ F cycle until stain satisfactory]^ [counterstain in any DS 11.21 

 formula if desired] -* balsam via usual reagents 



recommended for: pathological microglia. 



41.2 van der Stricht 1895 see F 1200.0000 van der Stricht 1895 



41.2 Welters 1891 see DS 21.212 Wolters 1891 



41.3 Histological Methods 



41.3 Foster 1934 20540b, 9:91 



reagents required: A. water 1, tannic acid 1, sodium salicylate 1; B. 3% ferric 



chloride 

 method: [sections] -* water —> ^ , 10 mins. — > wash — * iS, several m ins. —» wash —> 



counterstain, if required — > balsam, via usual reagents 

 recommended for: cell walls of apical meristem. 



41.3 Hogan test. 1883 Cole Cole 1883, 5 



reagents required: A. 10% ferric chloride in 90% ale; B. 2% pyrogallol in 90% ale. 

 method: [sections]^ 90% ale. -^ A, 2 mins. -^ 90% ale, rinse —> B, till sufficiently 



stained — » 90% ale, wash -^ water —<■ glycerol -^ M 10 mountant 

 recommended for: general histology. 



41.3 Hogan le.'<t. 1878 Marsh Marsh 1878, 71 



kkagents reqlired: .1. 2%^ ferric chloride in 90%, ale; B. 2% pyrogallol in 95% ale. 

 method: [sections] -* 90% ale — » /I, 2 mins. —>■ ale, rinse -^ B,2 mins. -* ale wash — * 



balsam, via usual reagents 

 recommended for: cartilage. 



41.3 Kenney 1928 1887a, 5:283 



reagents reqi ired: A. water 90, 40% formaldehyde 10, sodium sulfantimonate 1 

 method: [pieces] -^ A, 24 hrs. —>■ wash -^ [sections] 

 recommended for: reticulum fibers. 



