AMS 12.1-AMS 13.1 ACCESSORY METAL STAINING SOLUTIONS 615 



12.1 Steiner 1950 591b Tech. Bull, 20:489 



formula: abs. ale. 100, uranium nitrate 1, mastic 1 



12.1 Wallart 1935 428oa, 12:254 



formula: abs. ale. 30, pyridine 30, ether 30, ammonia 0.03 



12.1 Weber 1944 SWlG—auct. 4285a, 21 :4o 



formula: isopropanol 45, dioxane 45, water 10, 40% formaldehyde 20, acetic acid 2, 



formic acid 2, chloral hj-drate 5, cobalt nitrate 0.3 

 note: The acids are added immediately before use. 



13 Other Mixtures 



13.1 formulas 



13.1 Bolsi 1927 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 274 



formula: water 80, glycerol 20, ammonia 0.3 



13.1 Bolsi test. 1933 Cajal and de Castro Cajal and de Castro 1933, 261 



formula : water 60, acetone 20, pyridine 20, ammonia 4 



13.1 Cajal 1907 test. 1933 ips. Cajal and de Castro 1933, 290 



formula: 33% acrolein 10, water 90 



13.1 Cajal and de Castro 1933 Cajal and de Castro 1933. 261 



formula: hydrogen peroxide (3%) 100, oxalic acid to sat. 



13.1 David 1934 see ADS 12.1 David 1934 



13.1 Fontana 1912 7176,55:1003 



formula: water 100, tannic acid 5, phenol 1 



13.1 King 1937 1879, 38:362 



formula: water 30, ammonia 30, pyridine 30 



13.1 Loffler 1890 see DS 23.215 Loffler 1890 (sol. A) 



13.1 del Rio-Hortega 1918 21344, 15:165 



formula: water 100, ammonium bromide 1, tannic acid 3 



13.1 del Rio-Hortega 1932 test. 1933 Cajal and de Castro 



Cajal and de Castro 1933, 263 

 formula: water 30, ammonia 30, pyridine 30 



13.1 Saflford and Fleisher 1931 20540b, 6:43 



formula: water 100, picric acid 0.25, tannic acid 5, ferrous sulfate 7.5 



13.1 Tron 1924 2190, 13 



formula: water 100, neoarsphenamine 0.7, acetic acid 0.4 



13.1 Willard 1935 17510,78:475 



formula: water 40, 95% ale. 40, pyridine 20, chloral hydrate 2.5 



13.1 Zettnow 1891 23684, 11:689 



formula: water 115, tannic acid 5, antimony potassium tartrate 0.75 

 preparation: Dissolve the acid in 100 water at 60°C.; add the tartrate dissolved in 15 

 water. 



AMS 20 Solutions Used after Staining 



20.0 GENERAL OBSERVATIONS 



It is extraordinary how far the science of microtomy as it is seen in these formulas 

 has lagged behind the photographic methods w-hich it is supposed to resemble. (Whether 

 this resemblance is justified has already lieen (juestioned.) Photographic developers are 

 designed to reduce to metallic silver, particles of silver bromide which have been ren- 

 dered unstable through the absorption of photon energy. Neither silver bromide nor 



