S41.1 SOLVENTS AND OILS 629 



41.1 Weieert test. 1938 Mallory carbol-xylol — compl. script 



^ Mallory 1938, 98 



formula: xylene 75, phenol 25 



41 1 Zirkle 1930 19938, 71:103 



REAGENTS required: A. 5% ale; i?. 11% ale; C. 18% ale; D. 30% ale; E. 45% ale 90, 



n-butanol 10; F. 62% ale 80, n-butanol 20; G. 77% ale 65, n-butanol 35; H. 90% ale 



45, n-butanol 55; /. abs. ale 25, n-butanol 75; J. n-butanol 

 method: [water] -^ ^, 1-5 hrs. -^ B, 1-5 hrs. -^ C, 1-5 hrs. -^ D, 1-5 hrs - E, 1-5 hrs 



-^ F 12 hrs. -^ G, 1 hr. -> H, 1 hr. -^ /, 1 hr. -> J, several changes if necessary, tiU 



dehydration complete -^ [paraffin] ^ 



note: This is the well-known " Zirkle's butyl alcohol schedule for plant tissues. It is 



equally applicable to animal tissues. 



