M 11,1 MOUNTING MEDIA ^'31 



balsams to which objects can be trans- drated and cleared in the usual 



ferred only after they have been dehy- manner. 



M 10 Mountants Miscible with Water 



This section may be divided into three ration of Crustacea These media have the 



groups. M 11 contains conventional gum disadvantage tliat they must be melted 



arable media (of which Farrants' is the l)efore use and are therefore, not nearly 



tvoe) to which objects may be trans- as simple to use as the gum arable media, 



er'red eUher directly or from water. These The third group, M 13, which will prob- 



media should be far more widely used ably become more numerous as time goes 



Than is commonly the case, for a great on, employs water-tluckemng agents other 



deal of time is wasted in dehydrating and than gum arable or gelatin. Any one o 



intransferringtobalsamobjectswhichwere these three groups may be "^^^^d with 



better mounted in gum arable. The second stains for special purposes and the best 



group (M 12) contains glycerol jellies known media of this type are those of 



which are widely used by botanists and to Zirkle, some of which will be found in each 



a lesser extent by zoologists for the prepa- of the three sections. 



11 GUM ARABIC MEDIA 

 11.1 Formulas 



11 1 Allen test. 1937 Gatenby and Cowdry Gatenby and Cowdry 1937, 221 



' FORMULA- water 45, glycerol 11, 40% formaldehyde 4.5, gum arable 45 



pheparation: Dissolve gum arable in water. Mix formaldehyde m the glycerin and add 

 slowly, with constant stirring, to gum. 



11.1 Andre test. 1942 Langeron ^^^TT 9nn^' ^^^ 



formula: water 50, glycerol 20, gum arabic 30, chloral hydrate 200 

 preparation: Dissolve gum arabic in water. Mix glycerol in gum and add chloral 

 hydrate. 

 11.1 Apathy 1892 23632,89:1065 



formula: water 30, gum arabic 30, levulose 30 



preparation- Dissolve gum arabic in water. Add levulose to solution. 

 NOTE - Much grief in the prevention of bubbles may be avoided by reducing the water to 

 20 and using commercial levulose syrup in place of the dry sugar. 



■ "•' rr.: iriJ.T^ic acid 3, dextrose syrup 5, .urarawifcMora, hydrate 75 

 pheparation: Dissolve the acid in the water with the syrup and gum arabic. Add 



chloral hvdrate to the solution. 

 NOTE- Swan 1936 (4184, 27:389) states that Berlese first disclosed the formula to 

 Davidson in 1919, who communicated it to Lee by whom it was published m 1921. 

 Doetschman 1944 (21400a, 63:175) uses three times as much water in his formula lor 

 "15erlese." . • 



11 1 Brun 1889 see P 12.3 Brun 1889 

 11.1 Chevalier 1882 Chevalier 1882, 319 



formula: water 60, gum arabic 20, glycerol 20 

 11 1 Davies circ 1865 Davies, 82 



formula: water 30, gum arabic 30, glycerol 30, arsenic trioxide 0.1 



''•' F^RM^LATatef 35, glycerol 20, dextrose syrup 20, gum Irabiclo! 'chloral hydrate 20, 



sat. aq. sol. magenta 0.3 



11.1 Doetschman 1944 see also M 11.1 Berlese (note) 



11 1 Ewie test. 1944 Doetschman 21400a, 63 :175 , , , . on 



formula: water 35, glycerol 12, dextrose syrup 3, gum arabic 20, chloral hydrate 30 



