036 METHODS AND FORMULAS M 12.1-M 13.1 



12.1 Zirkle 1947 20540b, 22:87 



formula: water 45, acetic acid 30, lactic acid 15, Rolatin 10, orcein to sat. 

 PREPARATION : Dissolve the gelatin in water. Add other ingredients, boil 2-3 minutes. 



12.1 Zwemer 1933 Gluchrodd—auci. 763,57:41 



formula: water 80, gelatin 3, glycerol 20, chrome alum 0.2, camphor 0.1 

 preparation: Dissolve gelatin in 50 water with heat. Add glycerol to hot solution. Dis- 

 solve chrome alum in 30 water and add to liot mixture. Filter, then add camphor. 

 note: This formula was republished by Wotton and Zwemer 1935 (20540b, 10:21). 



13 OTHER WATER-MISCIBLE MOUNTANTS 



13.1 Formulas 



13.1 Archibald and Marshall 1931 16035, 23:272 



formula: water 60, gum tragacanth 0.5, ale. q.s., gum acacia 1.5, lactic acid 12, glycerol 



12, phenol 12 

 preparation: Add enough alcohol to gum tragacanth to make thin paste. Flood 10 

 water on paste with constant stirring. Dissolve gum acacia in 50 water. Mix with 

 tragacanth mucilage. Add acid, glycerol, and phenol to mixed gums. Filter. 

 RECOMMENDED FOR: wholcmounts of invertebrate larvae. 



13.1 Bernhardt 1943 1752,48:533 



formula: water 1, glycerol 2, phenol 1, lactic acid 1 



13.1 Downs 1943 19938, 97 :639 



STOCK solution: dissolve 15 polyvinyl ale. in 100 water at 80°C. 

 working formula: .stock 56, lactic acid 22, phenol 22 



note: The same formula was republished, with proper acknowledgment, by Huber and 

 Caplin 1947 (1829, 56:763) to whom the medium is often attributed. 



13.1 Huber and Caplin 1947 see M 13.1 Downs 1943 (note) 



13.1 Jones 1946 16730a, 21:85 



formula: water 35, polyvinyl ale. 6.3, sat. sol. picric in abs. ale. 18, lactophenol 45 

 formula: mix polyvinyl ale. to a paste with the picric solution. Add water to paste and 

 stir to jelly. Add lactophenol to jelly and heat to transparency on water bath. 



13.1 Gray and Wess 1950 11360, 70:290 



formula: water 30, lactic acid 15, glycerol 15, 70% acetone 20, polyvinyl ale. 6 

 preparation: Add the acetone slowly and with constant stirring to the dry resin. Mix 

 half the water with the lactic acid and glycerol and add to resin mixture. Add remain- 

 ing water slowly and with constant stirring. Heat on a water bath until clear. 



13.1 Monk 1938 19938,88:174 



formula: levulose syrup 35, pectin gel 35, water 20, thymol 0.1 



13.1 Monk 1941 21400a, 60:75 



formula: dextrose syrup 30, pectin gel 30, water 30 



note: Karo brand dextrose and Certo brand pectin are specified in the original of both of 

 Monk's formulas. 



13.1 Roudanowski 1865 11024,2:227 



formula: water q.s., isinglass 5, glycerol 8 



preparation: Leave isinglass in water and soak overnight. Drain and melt. Add glycerol 

 to molten material. 



13.1 Watkin 1925 see DS 21.41 Watkin 1925 



13.1 Zirkle 1937 19938,85:528 



formula: DS 11.23 Belling 1921 80, levulose syrup 10, pectin jeUy 10 

 note: The original specifies Karo brand levulose syrup and Certo brand pectin jelly. 



