E 11. 1 EMBEDDING MEDIA 643 



11.1 Belezky 1931 22575, 282:214 



REAGENTS REQUIRED: ^.. Water 75, phenol 7.5, gelatin 25; B. 4% neutralized formalde- 

 hyde 

 method: [small pieces] —> A, 2-3 hrs., 37°C. —» cool —> cut small blocks containing 

 pieces from mass -* B, 2-5 days — > [frozen sections] 



11.1 Blank and McCarthy 1950 11284, 36:776 



formula: Carbowax 4000 90, Carbowax 1500 10 



method: [fixed and washed tissues] —> water ^ "wax," 56°C. till impregnated —> 

 [block] 



note: The Carbowaxes (products of the Carbide and Chemical Corporation) are solid 

 polyethylene glycols. "1500" has about the consistency of petroleum jelly, "4000" 

 about that of 58° paraffin; other grades are available. All grades are water soluble, 

 which makes embedding easy, but it is very difficult to flatten ribbons before they 

 disintegrate. 



11.1 Brunottil892 11074,6:194 



reagents required: A. water 100, gelatin 10, acetic acid 15, mercuric chloride 0.5; 



jB. 5% potassium dichromate 

 method: [water] -^ 1 part A, 3 parts water, till impregnated —» A, till impregnated —> 



B, dropped in with as much A as possible adhering 



11.1 Bunge test. 1877 Frey Frey 1877, 71 



formula: egg white 72, 10% sodium carbonate 7.5, tallow 27 



preparation: Whip egg white and sodium carbonate together. Melt tallow and in- 

 corporate with egg white. 

 method: embed from water. Harden in alcohol 



11.1 Chatton 1923 6630,88:199 



formula: water 100, agar 1.3, 40% formaldehyde 2.5 



preparation: Dissolve agar in boiling water. Add formaldehyde to hot solution. 

 method: [object from water] —> surface of thin slab cast from medium —> pour just 



liquid medium on top, allow to set — * 95% ale. 

 note: This is primarily intended for setting and orienting small objects, the block being 



re-embedded in paraffin. 



11.1 Clark 1947 11431, 59:337 



reagents required: A. \2.b% gelatin; B. 2b% gelatin; C. 2% formaldehyde; D. 0.5%, 

 gelatin; E. 40% formaldehyde 



method: [pieces of formaldehyde fixed material] — > thorough wash —y A,24 hrs. 37.5°C. 

 — > B, 24 hrs. —^ [make block and refrigerate — trim block] -^ C, 24 hrs. — * [frozen sec- 

 tions] -^ 50% ale. -^^ D, few moments -^ place on clean slide -^ drain — > vapor from 

 E, 1 hr., 27°C. -^ C, till required 



recommended for: fatty tissues. 



note: a detailed description of this technique is given in Chapter 15. 



11.1 Cole 1884 Cole 1884, 39 



formula: water 24, gum arable 36, sugar 18, water 16 

 recommended for: use on freezing microtomes. 



11.1 Cutler 1935 1887a, 20:445 



reagents required: A. 95% ale. 50, glycol stearate 50; B. glycol stearate 



method: [fixed tissues] -♦ 95% ale, via graded series -^ A, 56°C., 12-24 hrs. -^ B, 24-48 



hrs. -^ [make block] 

 note: Ribbons may be attached to slide and processed exactly as paraffin ribbons. 



11.1 Flemming test. 1877 Frey Frey 1877, 71 



formula: hard soap 75, 95% ale. 25 



11.1 Frey 1877 Frey 1877, 71 



formula: isinglass 30, water q.s., glycerol 30 



preparation: Soak isinglass in water. Drain. Melt. Mix glycerol with molten isinglass. 

 method: embed from 50% glycerol. Harden in alcohol 



