644 METHODS AND FORMULAS E 11.1 



11.1 Gaskell 1912 see E 11.1 Romeis 1948 



11.1 Gerlach 1885 see M 12,1 Gerlach 1885 



11.1 Godfrin test. 1942 Langeron cit. Brunotte 1889 Langeron 1942, 440 



formula: water 25, glycerol 20, gelatin 2.5, 90% ale. 150, castor oil soap 50 

 preparation: Dissolve gelatin in water with glycerol on water bath. Mix oil in ale. and 



add to hot solution. 

 method: [woody tissues]-^ 95% ale. in vacuo to remove air — > water, some hrs. — > 



medium, freshly prepared — * continue heating till skin forms on surface — » withdraw 



object 



11.1 Graff 1916 see E 11.1 Romeis 1948 



11.1 Hartley 1938 Microscope, 2 :46 



formula: water 20, glycerol 40, gum arable 20, thymol 0.1 

 method: [bundles of textile fibers] — * medium, till saturated -^ dry — > section 

 RECOMMENDED FOR: transverse sections of textile fibers. 



11.1 Hermga and ten Berge 1923 23632, 40:166 



method: [object washed free of fixative] -> 10% gelatin, 2-5 hrs., 37°C. -^ 20%, gelatin, 

 37°C., 10 mins. — > [make block, cool] -^ [frozen sections] -^ sat. sol. thymol — > 

 attach to slides with V 21.2 Heringa and ten Berge 1923 -^ stain 



11.1 Kaiser 1880 see M 12.1 Kaiser 1880 



11.1 Langeron 1942 Langeron 1942, 439 



formula: water 60, gum arable 20, glycerol 20 

 RECOMMENDED FOR: use On freezing microtome. 



11.1 Lebowich 1936 1887a, 22 :782 



formula: 56°C. paraffin 80, stearic acid 20, diethylene glycol 1.5, ethanolamine 1.5 



preparation: Mix completely the molten waxes, preferably with high-speed electric 

 mixer. Add other reagents and continue mixing 30 minutes at 85°C. 



REAGENTS REQUIRED: A. acctone; B. soap from above 



method: [fixed material]—* A, till dehydrated^ B, 56°-62°C. (in vacuo for large ob- 

 jects), till impregnated-^ [cast in block and section] —> [mount sections by V 21.3 

 Lebowich 1936] 



11.1 Lubkin and Carsten 1942 19938, 97:168 



formula: water 80, polyvinyl ale. 16, glycerol 20 

 preparation: Dissolve the ale. with continuous stirring at 75°-85°C. Incorporate 



glycerol while cooling. 

 method: Place well-washed tissue blocks in medium in covered dishes. Heat to 56°C. 



for 2 hrs. daily. When solidified (8-9 days), trim and cut as though the block were 



nitrocellulose. 



11.1 Nicolas 1896 2844, 3 :274 



reagents required: A. 3% gelatin; B. 10% gelatin; C. water 90, gelatin 20, glycerol 10; 



D. 5% formaldehyde 

 method: [water] -> A, 1-2 days, 25°C. -^ B, 1-2 days, 25°C. -^ C, 2-3 days 35°C. -» 



[transfer to paper box, cool] -^ D, till hard enough to cut 



11.1 Pickworth 1934 11025, 69:62 



formula: water 100, phenol 1, gum arable 25 



11.1 Polzam test. circ. 1890 Francotte Francotte, circ. 1890, 287 



formula: dried hard soap 15, glycerol 35, 95% ale. 50 

 preparation: Boil ingredients with reflux to a syrupy mass. 



method: [objects too delicate for paraffin embedding]-* 95% ale. — > medium at 30- 

 40°C. — » [cool as block] — > [section] 



