646 METHODS AND FORMULAS E 21.1 



follows, therefore, the formulas which may be used to replace paraffin are given and also 

 some techniques by which any material may be embedded in any selected formula. 



21.1 Formulas 



21.1 Altmann test. 1942 Langeron Langeron 1942, 422 



formula: 60°C. paraffin 85, tristearin 10, beeswax 5 



21.1 Beyer 1938 591b, 2:173 



formula: paraffin 100, rubber 2, beeswax 0.5 



21.1 Bragg 1938 23639b, 28:154 



REAGENTS REQUIRED: A. water 20, 95% ale. 40, aniline 30; B. 95% ale. 30, aniline 60; 



C. aniline; D. aniline 50, toluene 50; E. toluene; F. sat. sol. 53° paraffin in toluene; 

 G. 53° paraffin 



method: [embryos in 70% ale] -^ A, 2-6 hrs. -^ B, 2-6 hrs. -^ C, \ hr. or till clear — > 



D, 1-6 hrs. -^ E, 1-3 hrs. -» F, 1-4 hrs. -^ G, 3-4 hrs., 56°C. -* [cast in block] 

 note: This is recommended for heavily yolked embryos. 



21.1 Debauche 1939 20540b, 14:121 



FORMUL.\: paraffin 80, beeswax 20 



21.1 Dufrenoy 1935 6630, 119:375 



method: [water] -^50% methylal, 30 mins. -> methylal, 30 mins. — > methylal 2, Uquid 

 petrolatum 3, 30 mins. -^ methylal 1, liquid petrolatum 3, 30 mins. — > liquid petro- 

 latum, 30°C., 30 mins. — >• [paraffin] 



21.1 Gray 1941 U.S. Patent 2,267,151 



formula: paraffin (MP 58°C.) JO, rubber 5, beeswax 5, spermaceti 5, nevillite "5" 



("clarite") 15 

 note: This composition melts at about 50°C. but will cut 5 m ribbons at a room tem- 

 perature of 85°F. By increasing the resin to 30 it is possible to cut 1 ju ribbons. 



21.1 Gudden test. 1895 Rawitz Rawitz 1895, 31 



formula: stearic acid 48, lard 48, beeswax 2 



21.1 Hance 1933 19938,77:353 



stock: Dissolve about 20 crude rubber, in small pieces, in 100 paraffin heated to smoking. 

 WORKING formula: paraffin 100, stock 4-5, beeswax 1 



21.1 Hetherington 1922 11428,9:102 



method: [nematodes] —> F 0000.0010 Hetherington 1922-^ methyl salicylate —> wax 



21.1 Johnston 1903 11032,6:2662 



formula: paraffin 99, asphalt 0.1, para rubber 1 

 preparation: Heat ingredients to 100°C. with occasional stirring for 48 hours. Decant. 



21.1 Hsii and Tang 1939a 20540b, 14:151 



formula: Japan wax 90, beeswax 10 



21.1 Hsii and Tang 1939b 20540b, 14:151 



formula: Japan wax 30, paraffin 70 



21.1 Larbaud 1921 see E 21.1 Peeters 1921 



21.1 Langeron 1942 Langeron 1942, 415 



method: [water] -^95% ale. -^ amyl ale. 24 hrs. -^ liquid petrolatum, 2-3 changes in 

 24 hrs. — > [paraffin] 



21.1 Maxwell 1938 20540b, 13:93 



formula: paraffin 56-58° 100, rubber paraffin (see E 21.1 Hance 1933) 4-5, bayberry 

 wax 5-10, beeswax 1 



