648 METHODS AND FORMULAS E 22.1 



22.1 Espinasse test. 1937 Gatenby and Painter Gatenby and Painter 1937, 96 



REAGENTS REQUIRED: A. methyl benzoate 100, celloidin 1 

 method: abs. ale. -^ A, till permeated -^ benzene — > paraffin 



22.1 Field and Martin 1894 23632, 11:6 



method: Dissolve celloidin moistened with toluene in a mixture of equal parts toluene 

 and abs. ale. to give a thick solution. Warm to 25°C. and add, little by little, as much 

 paraffin as the solution will take up. Pass object to mass from equal parts toluene and 

 abs. ale. When impregnated transfer to a sat. sol. paraffin in chloroform. Evaporate 

 solvents at 56°C. and treat block as paraffin block. 



22.1 Heinz 1923 14674,70:913 



REAGENTS REQUIRED: A. abs. alc. 50, ether 50; B. methj'l salicylate 50, ether 25, abs. 



ale. 25, celloidin 1; C. chloroform; D. sat. sol. paraffin in chloroform 

 method: [pieces from abs. ale] -^ A,\2 hrs. — > B, 24 hrs. -^ C, 12 hrs. -^ D, 12 hrs. — > 



[paraffin] 



22.1 Jordan and Heather 1929 20540b, 4:121 



formula: abs. alc. 25, ether 25, methyl salicylate 25, celloidin 1 



method: [fixed and dehydrated pieces] — > 50:50 ether abs. alc, 8 hrs. -^ celloidin mix- 

 ture 24 hrs. -^ sat. sol. paraffin in chloroform 2-3 hrs. — > paraffin, 2 changes, till 

 permeated -^ [make block] 



22.1 Peterfi 1921 23632, 38:342 



FORMUL.\: methyl benzoate 100, collodion 1 



method: [abs. alc] — > methyl benzoate —> medium, till perfectly clear —> cedar oil — > 

 paraffin 



22.1 Reichardt and Wetzel 1928 23632, 45 :476 



reagents required: A. methyl benzoate; B. 1% celloidin in methyl benzoate; C. 10% 



paraffin in methyl benzoate; D. paraffin (50°C.) 

 method: [objects to be embedded] — > abs. alc. till perfectly dehydrated — > A, till clear 



-^ B, 3-5 days -^ C, 1-2 days, 40°C. -^ D, 1 day -^ [make block] 



22.1 Richardson 1934 11571b, 13:81 



formula: celloidin 110, abs. alc. 25, ether 110 

 PREPAR.\TiON : Leave celloidin in alc. overnight. Mix ether in alc. solution and leave 



several daj^s. 

 method: [formol fixed material] —> acetone 2 hrs. — > clove oil, till clear -^ celloidin, 6 



hrs. — » [prepare block in usual manner] 



22.1 Seki 1937a 23639b, 27:278 



method: [dehydrated object] — > methanol, 24 hrs. — > equal parts methanol and ether, 

 12 hrs. —* 1% celloidin in methanol 12 hrs. — > [evaporate to ?^rds volume] -^ chloro- 

 form, 24 hrs. -^ anhydrous butyl alc, 12-24 hrs. -^ benzene, 3-5 hrs. — > sat. sol. 

 paraffin in benzene, 35°C., ^ 2~1 hr. —>■ paraffin 



22.1 Seki 1937b 23639b, 27:282 



method: [dehydrated object] —» 2% celloidin in methanol, 1 day — >• 4% celloidin in 

 methanol, 2 days -^8% celloidin in methanol, 4 days -^ [set in block] -^3% chloro- 

 form in 70% alc, 1 day -^ section 



22.1 Stepanow 1900 23632, 17:185 



REAGENTS REQUIRED: A. clovo oil; B. ether 80, abs. alc 4, clove oil 20, celloidin 6 

 method: [dehydrated tissues] —> A, 3-6 hrs. — > B, 3-24 hrs. -^^ [cast block, allow to 

 evaporate until transparent] — > chloroform 



22.1 Tschernyachinsky 1930 23632,47:200 



method: [objects impregnated with 8% celloidin in abs. alc.-ether] —* equal parts 8% 

 celloidin and clove oil, 2-24 hrs. — » chloroform, 30 mins. — ^ sat. sol. paraffin in chloro- 

 form 56°C. 1 hr. -^ paraffin 



