V 21.1 VARIOUS FORMULAS G57 



without regani to the specifications given by the inventor: the material may be first 

 smeared on the shde, or it may be (hhited considcr;d)ly witli water and tliis (hluted ad- 

 hesive used to flatten the section. It may be tliought curious that formulas for label ad- 

 hesives should be included, but there is certainly no commoner reason for the loss of a 

 valuable preparation than the detachment of the label. It is warmly recommended that 

 one of those formulas be employed which contain a small quantity of glycerol for the 

 purpose of preventing the absolute liardening of the adhesive. The entire label and not 

 only the adhesive upon it should be moistened to avoid a differential contraction in dry- 

 ing, which invariably strips the adhesive from the glass. 



21 FOR ATTACHING SECTIONS TO SLIDES 

 21.1 For Attaching Paraffin Ribbons 

 21.1 Artschwager 1919 see V 21.1 Szombathy 1918 (note) 



21.1 Bohm and Davidoff 1905 Japanese method — compl. script. 



Bohni and Davidoff 1905, 30 

 REAGENTS REQUIRED: A. V 21.1 Mayer 1884; B. 0.5% gum arabic 

 method: slides are very thinly coated with A and then dried at 70°-80°C. to coagulate 



albumen. Sections are flattened on the slide with warm B. 

 note: In European literature, other than English, this method is fairly universally 

 known as the "Japanese method" (cf. Cajal and de Castro 1933, 65; Spielmeyer 1924, 

 48) 



21.1 Claoue 1920 ted. 1942 Langeron Langeron 1942, 481 



formula: alcohol 50, ether 50, pyroxylin 0.4, castor oil 3, camphor 2 

 preparation: Dissolve alcohol, ether, and pyroxylin. Add castor oil and camphor to 



solution. 

 method: applied at any time from a drop bottle. The slide should be blotted free of 



excess reagent before the adhesive is applied. 

 recommended for: cementing in place sections which have loosened 



21.1 Cobe and Schoenfle 1946 Tech. Bull., 7:31 



preparation: In 100 boiling 0.2% potassium dichromate dissolve 0.02 gelatin. Boil 5 



minutes, cool, filter. 

 method: flatten ribbons on bath of warm solution, strand on slide, and dry. 



21.1 Crabb 1935 19938,80:530 



reagents required: A. colloidin USP 40, abs. ale. 20, ether 20, amyl acetate 20; 



B. collodion USP 50, abs. ale. 25, ether 25 

 method: [ribbons, spread and flattened on slide and thoroughly dried] -^ A, flooded on 



slide, 30 sees. -> blot -^ B, flooded on slide —>■ drain -^ dry -^70% ale, 5 mins. -^ 



95% ale, quick wash — * xylene 



21.1 David 1935 19938, 82:179 



formula: water 100, "waterglass" 1, ammonia 1 



21.1 Gravis 1889 4992, 15:72 



formula: water 100, agar 0.1, camphor 0.1 

 preparation: Dissolve agar in water with boiling. FUter. Add camphor to filtrate. 



21.1 Haupt 1930 see V 21.1 Szombathy 1918 (note) 



21.1 Heidenhain 1905 23632,22:331 



formula: water 75, albumen 2, 95% ale. 25 



preparation: Dissolve albumen in 50 water. Filter. Mix 95% ale. with 25 water. Add 

 to filtered solution. 



21.1 Hollande 1911 1823,13:171 



formula: garlic 50, water 80, chloroform 1 



preparation: Crush ingredients. Triturate. Leave 24 hours. Filter. 

 note: For some reason this appears to work better than a pectin solution of comparable 

 strength. 



