G58 METHODS AND FORMULAS V 21.1 



21.1 Land test. 1915 Chamberlain Chamberlain 1915, 114 



REAGENTS REQUIRED: A. 1% gum arabic; B. 0.5% potassium dichromate 

 METHOD : Smear A on slide. Flood with B. Warm to flatten sections. Drain, dry. 



21.1 LUlie 1945 20540b, 20 :99 



formula: water 100 dried egg albumen 5, sodium chloride 0.5, glycerol q.s., 0.01% 



merthiolate 0.5 

 preparation: Shake the water, albumen, and sodium chloride gently together till dis- 

 solved. Philter. Add glycerol to make double volume of filtrate. Add merthiolate to 

 mixture. 



21.1 Masson 1928 608b, 4:181 



reagents required: A. 0.25% gelatin; B. 40% formaldehyde 



method: flatten sections on A. Drain. Expose to vapor of B at 50°C., 20 mins. to 

 overnight. 



21.1 Masson test. 1942 Langeron Langeron 1942, 476 



reagents required: A. 1% gelatin; B. 95% ale. 80, 40% formaldehyde 20 

 method : [paraffin ribbons] — > float on A and warm to flatten — * drain -^ dry heat to 

 melt paraffin -^ xylene, till wax removed — * abs. ale. -^ B, 5 mins. — > 50% ale. 



21.1 Mayer 1884 11360,4:317 



formula: fresh egg white 50, glycerol 50, sodium salicylate 1 

 preparation: Agitate ingredients at intervals for some days. Filter. 

 recommended for: spreading in thin layer on slide; or, diluted 20: 1 with water, 

 flattening sections. 



21.1 McDowell and Vassos 1940 1789a, 29:432 



formula: water 90, starch 3, 10% hydrochloric acid 0.5, thymol 0.1 

 preparation: Mix starch in 30 water to make a smooth paste. Raise 60 water to boiling. 

 Add to starch paste. Add hydrochloric acid to starch. Boil 5 minutes. Cool. Add thymol 

 to cooled starch. 



21.1 Moreau 1918 5293, 34:164 



REAGENTS REQUIRED: A. 0.01% gelatin, freshly prepared; B. 90% ale. 80, 40% formal- 

 dehyde 20 

 method: sections are flattened on warm A and dried. After passage through benzene 

 and abs. ale, B is applied from a drop bottle. 



21.1 Regaud test. 1942 Langeron Langeron 1942, 481 



REAGENTS REQUIRED: A. coUodiou USP 20, ether 40, abs. ale. 40; B. 70% ale. 

 method: [sections attached to slide but apparently loose] -^ abs. ale. —* A, 2 mins. — » 



drain —>£,—* stain, etc. 

 recommended for: cementing in place paraffin sections which appear loose after 

 dewaxing. 



21.1 Reinke test. 1928 Schmorl Schmorl 1928, 77 



formula: egg albumen 50, glycerol 50 



preparation: Beat egg white until stiff. Leave till the mass has reverted to a clear 

 fluid. Filter and add an equal volume of glycerol. 



21.1 Riiyter 1931a 23632, 47 :226 



formula: water 80, V 21.1 Mayer 1884 1, acetone 20, methyl benzoate 0.3 

 preparation: Mix 21.1 Mayer 1884 with water. Mix acetone and methyl benzoate and 



add to first mixture. 

 method: use to float and flatten ribbon 



recommended for: attaching ribbons from double-embedded (nitrocellulose-paraffin) 

 blocks. 



21.1 Riiyter 1931b 23632, 48 :226 



formula: water 80, acetone 20, methyl benzoate 0.6 

 method: flood under ribbons on slide. Warm. Dry. 

 use: as Riiyter 1931a 



