V21.1-V21.3 VARIOUS FORMULAS 050 



21.1 Schneidau 1937 21559,56:258 



formula: water 100, e^;g all)uin(Mi 0.0(1, ulycorol l.G, sodium salicylate O.OG 

 KECOMMENDKi) FOR: iiattriiiiig ami fixing i)arallin rihhoii.s. 



21.1 Spoerri 1939 199;i8, 90:200 



formula: water 100, starch 3, hydrochloric acid 0.2 



preparation: Suspend the starcli in ;30 water; add 70 boiling water and stir. Add acid 

 and boil 5 minutes. 



21.1 Szombathy 1918 230:52,34:3:34 



REAGENTS REQUIRED: A. Water 100, gelatin 1, glycerol 15, .sodium salicylate 0.2; B. water 



98, 40% formaldehyde 2 

 method: coat slide thinly with .4. Flood with B on which sections are flattened in tlie 



usual manner. Dry. 

 note: This method is usually known to botanists from a paper ])y Artschwager 1919 



(3430, 67:373). Haupt 1930 (205401), 5:97j substitutes 2 pheuortor the salicylate in 



A above. 



21.2 For Attaching Sections in Nitrocellulose 



21.2 Heringa and ten Berge 1923 23632, 40:166 

 REAGENTS REQUIRED: A. 3% gelatin; B. 5% sodium sulfate 

 method: [coat slides with A] — >• dry —> soak in B, 2 hrs. —> wash --> dry 



21.2 Langeron 1942a Langcron 1942, 482 



rea(;ents required: A. V 21.1 Mayer 1884; B. oil of cloves; C. abs. ale; D. abs. ale. 50, 



ether 50 

 method: [section from 70% ale] — > press to slide coated with A —* blot with consider- 

 able pressure — > B, from drop bottle, few mins. -^ C, in jar, 15 mins. —> D, till nitro- 

 cellulose dissolved — > abs. ale. — > stain, etc. 



21.2 Langeron 1942b Langeron 1942, 483 



reagents required: A. water 100, gelatin 10, phenol 0.5; B. 4% formaldehyde; C. abs. 



ale; D. abs. ale. 50, ether 50 

 method: [section from 70% ale] — > press to slide coated with A —> blot thoroughly -^ 



B, 5 mins. —> C, 2-3 mins. —^ D, till nitrocellulose dissolved — > abs. ale — > [stain, etc.] 



21.2 Linstaedt 1912 763, 6:445 



reagents required: .1. water 100, dextrin 3, sucrose 3; B. 4% celluloid in acetone; 



C. 1% celloidin in ether-ale 



method: coat glass plates with A and dry; spray or dip coat with B; store till required. 

 Arrange sections on water film on coated plate; blot; spray with C. When partially 

 dry dip in 70% ale, then in water till celluloid sheet bearing sections can be detached. 



recommended for: preparation of sheets of sections which may be cut up with scissors, 

 after staining, for issue to classes. 



21.2 Riiyter 1931 see V 21.1 Rliyter 1931 



21.3 For Attaching Individual Sections Requiring Staining 



21.3 Altmann 1894 tcsl. 1905 Bohm and Davidoff Bohm and Davidof!" 1905, 78 

 reagents required: A. 3% gutta percha in chloroform; B. collodion USP 

 method: Slide is coated with A, dried and stored. When warmed, coated slides liecome 



tacky. Sections are pressed to tacky surface, cooled, and varnished with B. 



21.3 Chiovenda 1936 test. 1937 Foot 4349, 17:173 



formula: water 84, V 21.1 Reinke (1928) 12, glycerol 4, merthiolate 9.5 

 method: soak sections in adhesive; strand on clean slide; drain; dry at 30-40°C.; place 

 in abs. ale 



21.3 Fol test. 1937 Gatenby and Cowdry Gatenby and Cowdry 1937, 116 



formula: water 72, 95% ale 30, acetic acid 7, gelatin 1.5, chrome alum 0.1 

 preparation: Dissolve acetic acid in gelatin with heat. Mix 95% ale in 70 water and 



add to solution. Dissolve chrome alum in 2 water and add to mixture. 

 method: clean slides are dipped and dried. When again moistened, the surface becomes 



sticky without dissolving. 



