GGO METHODS AND FORMULAS V 21.3-V 22,1 



21.3 Heringa 1924 6630, 91:931 



REAGENTS REQUIRED: A. 3% gelatin; B. 2.5% sodium sulfate 



method: [clean slides] -^ A, dip -^ dry -^ B, I hr. —^ water, thorough wash -^ dry 

 note: Slides so treated are soaked for a few moments in water before having sections 

 pressed to them. 



21.3 Langeron 1942 Langeron 1942, 678 



formula: water 81, gum arable 30, aluminum sulfate 0.6 



preparation: Dissolve gum arable in 75 water. Dissolve aluminum sulfate in 6 water 

 and add to gum solution. 



21.3 Lebowich 1936 1887a, 22 :782 



reagents required: A. 0.5% gelatin in 1% (of "3%") hydrogen peroxide; B. 2% 



neutraUzed formaldehyde; C. 2.5% gum arable 

 method: Dip chemically clean slide in A. Drain and dry. Dip in B. Drain and store. Use 



C to float and flatten sections. Drain, dry section in place. 

 note: This technique was originally intended for use with E 11.1 Lebowich 1936. 



Moritz 1939 (20540b, 14:17) recommends 0.25% gelatin in A. 



21.3 Masson test. 1942 Langeron Langeron 1942, 457 



REAGENTS REQUIRED: A. 70% alc; B. 70% ale. 50, ether 50; C. abs. ale. 50, ether 50, 



pyroxylin 0.25; D. 80% alc. 

 method: [sections floating in A] -^ strand on slide -^ B, from drop bottle-^ C, in jar, 



cautious dip — > drain — * D 



21.3 Moritz 1939 see V 21.3 Lebowich 1936 (note) 



21.3 Obregia 1890 15058,9:295 



REAGENTS REQUIRED: A. dcxtrose syrup 60, dextrin syrup 20, 95% alc. 40; B. 2% 



celloidin 

 method: Coat slides with A, very thinly. Dry slowly. Flatten section on sticky surface. 



Heat to 60°C. Remove paraffin with solvent. Remove solvent with abs. alc. Pour on 



B. Dry. Strip film with sections attached. 

 note: The strength of syrup is not important. The consistency should be about that of 



the simple syrup of the pharmacopeia. 



21.3 Schallibaum 1883 1780, 22 :689 



formula: 2% pyroxylin 20, oil of cloves 80 

 method: [make thin layer on slide] -^ press on section -^ dry 10 mins. -^ heat to 50°C. 



21.3 Windeholz 1923 7276, 70:877 



formula: "waterglass" 30, water 70 



method: spread a thin film on slide; leave till just dry; strand section on film. Blot and 

 leave 15 mins. 



note: "Waterglass" is a commercial solution of sodium silicate of very variable com- 

 position. 



21.3 Zimmermann 1896 Zimmermann 1896, 40 



REAGENTS REQUIRED: A. V 21.1 Gravls 1889; B. collodion USP 5, abs. alc. 25, ether 75 

 METHOD : sections -^ flattened on slide using A -* drain -^ dry -^ varnish in place with 

 B 



22 FOR ATTACHING WHOLE OBJECTS TO SLIDES 



22.1 For Objects Requiring Further Manipulation 



22.1 Chatton and Lwoff 1930 6630, 104 :834 



formula: water 100, gelatin 10, sodium chloride 0.05 



RECOMMENDED FOR: attaching fixed protozoans on slide before silver staining. Use at 

 25°C. 



22.1 Giesbrecht 1881 23833,4:255 



REAGENTS REQUIRED: A. 1% shellac to 95% alc; B. clove oil 



method: Coat slides thinly with A. Dry. Moisten with B. Arrange objects on oil film 

 and then evaporate oil at 60°C. 



