662 METHODS AND FORMULAS V 31.1 



tassium dichromate, may be used to fill the very finest capillaries with an opaque yellow 

 pigment. This is one of the oldest known methods of injection and cannot be equaled by 

 any of the more modern substitutes. 



31 FOR INJECTION AT ROOM TEMPERATURE 



31.1 Formulas 



31.1 Altmann 1878 test. 1893 Schaffer 23632, 10:191 



formula: olive oil 50, 95% ale. 25, ether 25 



method: small pieces of decalcified bone are impregnated in vacuo, washed in water, 

 stained in 1% osmic acid for 24 hrs., and then sectioned. 



31.1 Beale 1880a Beale 1880, 111 



formula: water 30, 95% ale. 15, acetic acid 0.5, glycerol 60, ammonium hydroxide 0.3, 



carmine 0.6 

 preparation: Dissolve the carmine in 5 water with the ammonia. Mix the acetic acid 

 with 30 glycerol. Add this, with constant stirring, to the carmine solution. Mix 30 

 glycerol, 15 ale, and 25 water. Add this to carmine-acid mixture. 



31.1 Beale 1880b Beale 1880, 109 



formula: water 123, 95% ale. 30, glycerol 12, potassium ferrocyanide 0.8, ferric chloride 



0.6 

 preparation: Dissolve the potassium ferrocyanide in 24 water with 6 glycerol. Dissolve 



ferric chloride in 24 water with 6 glycerol. Add slowly, with constant agitation, to 



the ferrocyanide solution. Mix ale. in 75 water. Add to blue solution slowly and with 



constant agitation. 



31.1 Beale 1880c Beale 1880, 111 



formula: water 32, 95% ale. 10, glycerol 45, carmine 0.4, ammonium hydroxide 0.2, 



acetic acid 0.3 

 preparation: Mix carmine with water to make paste. Add ammonia to dissolve paste. 



Add 15 glycerol to carmine solution. Mix 15 glycerol with acetic acid. Add slowly, with 



constant stirring, to carmine. Add 15 glycerol, ale, and 32 water to mixture in order 



given. 



31.1 Beale 1880d Beale 1880, 363 



formula: water 30, glycerol 60, potassium ferrocyanide 0.2, ferric chloride 0.13, hydro- 

 chloric acid 0.1 

 preparation: Dissolve potassium ferrocyanide in 30 glycerol. Dissolve ferric chloride in 

 30 glycerol and add slowly, with constant agitation, to the ferrocyanide. Mix hydro- 

 chloric acid with water. Add slowly, with constant agitation, to the blue mixture. 



31.1 Bensley 1929 590,40:146 



preparation of stock solution: Dissolve 0.4 silver nitrate in 10 water. Add 5% 



sodium phosphate drop by drop until no further ppt. forms. Wash by deeantation. 



Accumulate ppt. in 3 parts water. Add 2.8 citric acid to wet ppt. Dilute with water 



to 100. 

 preparation of working solution: stock 25, 1% sodium citrate 75 

 method: inject after washing out blood with sodium citrate. Fix injected pieces in 4%, 



formaldehyde. Cut frozen sections and develop with any AMS 21.1 formula. 



31.1 Brucke 1865 1780, 1 :87 



formula: 2.17% potassium ferrocyanide 20, 12% sodium sulfate 80, 10% ferric chloride 



20 

 preparation: Mix 40 sodium sulfate with potassium ferrocyanide. Mix 40 sodium 

 sulfate with ferric chloride. Add to above solution slowly and with constant stirring. 



31.1 Doyere 1841 test. Cooper 1847 Cooper 1847, 156 



REAGENTS REQUIRED: A. solutiou of potassium chromate; B. solution of lead acetate 

 method: inject A through an artery until it runs from a vein. Then inject B. 



