664 METHODS AND FORMULAS V 31.1-V 32,1 



31.1 Seller 1881 Seller 1881, 78 



formula: water 100, gelatin 4.5, silver nitrate 0.2 



preparation: Add the silver dissolved in 35 water to the gelatin dissolved in 65. 

 method: drain blood vessels and fill with medium. Develop in any AMS 21.1 formula. 

 RECOMMENDED FOR: demonstration of endothelium of blood vessels. 



31.1 Tandler 1901 23632,18:22 



formula: water 100, gelatin 5, potassium iodide 6 



preparation: Melt gelatin in water. Add potassium iodide to hot mass. Cool. 

 note: Pearl 1902 (11032, 5:1736) is essentially the same. 



31.1 Thoma 1899 1739,74:270 



formula: water 60, glycerol 40, indigo-carmine 0.15, sodium chloride 1 

 PREPARATION : Dissolve indigo-carmine in 50 water. Add glycerol to solution. Dissolve 

 sodium chloride in 10 water. Add slowly to mixture with constant agitation. 



31.1 Hagmann 1940 20540b, 15:115 



REAGENTS REQUIRED: A. Water 90, acetic acid 10, Santomerse no. 3 1, trypan blue 2; 



B. water 75, 40% formaldehyde 10, acetic acid 15, barium chloride 40 

 method : chloroformed insects are placed under vacuum in a device which permits their 

 being dropped into A in vacuo. After 15 mins. the pressure is released and the insects 

 transferred to B for not less than 3 hrs. Specimens may be stored in 70% ale, mounted 

 in balsam, and sectioned by celloidin or paraffin techniques. Santomerse is a wetting 

 agent. 

 recommended for: injection of insect trachea. 



32 FOR INJECTION WARM 



32.1 Formulas 



32.1 Bensley test. 1929 Moore cit. Knouff 4349, 12 :55 



PREPARATION OF COLLOIDAL CARMINE: Dissolvc 40 cariuine in 100 water with 40 am- 

 monia. Leave mixture 12-24 hours. Filter. Boil filtrate until ammonia-free. Add 95% 

 ale. in excess to filtrate to precipitate carmine. Filter. Dry ppt. 

 WORKING mass: Dissolve carmine from above in 50 water. Soak 25 gelatin in 25 water. 

 Melt and mix with carmine. 



32.1 Carter 1862 Arch. Med., 3 :2S7 



formula: water 90, acetic acid 5, gelatin 12, ammonia 5, carmine 6 

 preparation: Dissolve carmine in ammonia. Melt 9 gelatin in 60 water. Mix with 



carmine. Dissolve 3 gelatin in 30 water with acetic acid. Add slowly with constant 



agitation to hot carmine-gelatin mixture. 



32.1 Fol 1883 23635,38:492 



STOCK i: Soak 100 gelatin in water. Drain. Melt. 

 stock ii: Warm carmine in 75 water with 25 ammonia. Leave overnight. Filter. Add 



acetic acid drop by drop until color changes to bright red. 

 preparation of stock mass: Stock I 100; stock II 100. Add the carmine solution to the 



molten gelatin. Stir well. Chill and shred the chilled mass. Wash the shreds in running 



water overnight. Drain, remelt, and cast in sheets. Dry sheets. 

 preparation of working mass: Soak stock mass in enough water to cover. Soak 10 



minutes. Drain. Melt. 



32.1 Fol 1884 Fol 1884, 13 



preparation of stock mass: Soak 50 gelatin in sheets in V 32.1 Fol 1883, stock II for 



48 hours. Drain. Rinse. Transfer rinsed sheets to 100 0.1% acetic acid solution. Leave 



overnight. Wash 3 hours in running water. Dry. 

 preparation of working mass: As Fol 1883 



32.1 Frey 1877 Frey 1877, 112 



formula: water ^..s., gelatin 25, sat. aq. sol. {circ. 40%) barium chloride 50, sulfuric acid 



o s 

 preparation: Add acid to barium chloride until no further ppt. produced. Soak gelatin 



in water. Drain. Melt and incorporate with barium sulfate. 



