760 



INDEX 



Oxidation-reduction, as fixative process, 188 

 Oxner's fixatives, mercuric-acetic, 209, 249 

 osmic-dichromate-acetic, 204, 249 

 osmic-dichromate-formic, 204, 249 



von Pacaut's fixative, 206, 249 

 Pacini's preservative, 180 

 Painter's fixative, 227 

 Painter, see also Gatenby, 176, 628 

 Pal's differentiator, 520 

 in, 



Anderson's method for neuroglia, 412; 

 Heller-Robertson's method for myelin 

 sheaths, 529; nerve staining techniques, 

 404-409; Perdrau's method for reticu- 

 lum, 594; Reich's method for granules 

 in Schwann cells, 457; Schaffer's method 

 for fat, 449 

 Pal on fixing spinal cord, 398 

 Paley, see Broadhurst, 490 

 Palladium chloride, as ingredient of, 



Choquet's decalcifying fluids, 259; 

 Frenkel's fixative, 205; Katz's decalci- 

 fying fluid, 259; Pampel's preservative, 

 177 

 Pancreas, dissection of, 439 

 mitochondria in, 439-441 

 paranuclear bodies in, 457 

 special methods for, 428, 429, 430 

 Pancreatin, as ingredient of macerating 

 fluids, 264 

 in Wall's method for reticulum fibers, 424 

 "Panoptic" stain, 318, 343, 349 

 Pantin on heating fixatives for invertebrate 



larvae, 187 

 Papanicolaou's methods for vaginal smears, 



431, 432 

 Papara, see Dotti, 252 



Paper boxes for, nitrocellulose embedding, 

 144ff, 150 

 paraffin embedding, 102ff, 104ff, 105ff 

 Paper cells for dry wholemounts, 12 

 Paper supports for fixing chicken embryos, 



277 

 Pappenheim's, fixative, 197, 249 

 method for, acid-fast bacteria, 477 



blood, 419 

 stains, methyl green-pyronin, 355 



methylene blue-azur Il-eosin Y, 349, 

 350 

 Paquin and Goddard's stains, hematoxylin- 

 anilin blue-eosin-orange G-phloxine, 

 366 

 iron-hematoxylin, 286 



in Paquin and Goddard's technique, 366 

 Parabenzoquinone, in Baker's method for 



mitochondria, 441 

 "Paracarmine," 301 



Parachlorophenol, as ingredient of, Amann's 



dehydrating mixtures, 628 

 Paradiaminobenzene hydrochloride, as in- 

 gredient of Holmes's developer, 617 

 Paraffin, as ingredient of, 



Apdthj^'s cement, 655; Belling's cement, 

 655; embedding waxes, 646-647; Hood 

 and Neill's cement, 656; Langeron's wax 

 for dishes, 667; Lataste's cement, 656; 

 Lebowich's soap embedding medium, 

 644 

 miscibility with, 



essential oils, 624; synthetic clearing 

 agents, 626; "universal" solvents, 626 

 properties of, 97 

 Paraffin blocks, action of knife on, 1 lOff 

 casting, 102, 103, 106ff, 107ff, 130 

 casting large, 139 

 choice of microtome for, 103 

 cooling, 103, 107, 135 



effect on cutting, 120, 121 

 cutting hard materials in, 122 

 cutting ribbons, 114ff, 115ff 

 effect of, faulty impregnation, 121, 122 



using wrong wax, 121 

 lift ribbon when cutting, 123 

 making card box for, lOOff, 10 Iff, 102 

 making paper boxes for, 102ff, 104ff, 105ff 

 method of orienting objects in, 135 

 mounting and trimming, 112, 113ff 

 softening specimens in, 667 

 storing, 103 



trimming with saw, 335 

 Paraffin embedding, impregnation of object, 

 100-101 

 infiltration methods, 99, 129, 130 

 of, embryos for freehand sections, 90 



nitrocellulose blocks, 155 

 orienting frog's eggs during, 135 

 ovens for, 98ff, 99ff, 106ff 

 selection of, 



clearing agent, 96; dehydrant, 96; wax, 

 97 

 technique of clearing before, 98 

 technique of dehydrating before, 97 

 technique with large objects, 139 

 transfer from solvent to wax, 99, 100 

 Paraffin-nitrocellulose ribbons, Riiyter's ad- 

 hesive for, 658 

 Paraffin ribbons, see under Paraffin embed- 

 ding and Paraffin sections 

 Paraffin sections, adhesives for, 657-659 

 attaching to, each other, 279 



slide, 131 

 cause of, failure to ribbon, 123 



failure to stain, 127 

 checking adhesion to slide, 131 

 cleaning, 125 



cutting on, rotary microtome, 114ff, 115ft' 

 sliding microtome, 140 



