PREFACE TO THE TENTH 

 EDITION 



In this new edition an important change has been made by the 

 inclusion of plant technique. This section has been written by Dr, 

 D, G. Catcheside, of London University, but has necessitated leaving 

 out two sections previously included in the ninth edition. The extension 

 of usefulness of the book which the inclusion of a section on plant 

 technique will effect, should more than make up for this sacrifice. It is 

 certain that the botanist or zoologist will benefit by looking over various 

 chapters describing the techniques in the other sister science, and thus 

 the new departure of putting both plant and animal micrology between 

 the same two covers will have been justified. 



New chapters now included are those on the frozen section technique, 

 and on vital staining. The latter has been written by Dr. R. J. Ludford, 

 whose great assistance and helpful advice throughout is gratefully 

 acknowledged. In this edition, Dr. H. J. Conn, the leading American 

 authority on staining, has recast the whole chapter on this subject, 

 and Dr. E. S. Duthie has done the same for the article on blood and 

 glands. Mr. K. C. Richardson has been responsible for the section on 

 celloidin imbedding, and the chapter on tissue culture, and Dr. Kay and 

 Dr. Whitehead have completely re-written the chapter on fats. Dr. 

 Helen Pixell-Goodrich has again been responsible for the article on 

 protozoological technique. 



Perhaps the most dilficult section in the book is that devoted to the 

 nervous system ; Dr. J. G. Greenfield and Dr. Ruby O. Stern undertook 

 the necessary revision at considerable inconvenience to themselves. 

 Their valuable collaboration has been especially helpful, and puts us 

 deeply in their debt. 



The ninth edition was going to press when the death of Arthur Bolles 

 Lee was announced, and there was not time to make more than passing 

 reference to his death, as well as to that of William Bayliss and of 

 C. Da Fano, two previous contributors. It is fitting that some account 

 of Arthur Bolles Lee should be given in this work. The Vade-Meeum 

 appeared first in 1885, when he was thirty-six years of age, and during 

 the time that he was an assistant at the Russian laboratory at 

 Villefranche, near Nice. Lee subsequently brought out seven editions 

 of his book, the last in 1913, when he was sixty-four years old. The 

 eighth edition appeared in 1921, Lee sending the present senior editor 

 300 references. The ninth edition ajjpeared in 1928. Lee was born at 

 Coldrey House, in Hampshire, hctwecn Farnham and Alton, in 1849, 

 and when he was eighteen he was articled to a London solicitor. On 

 his mother's death in 1871 his inheritance enabled him to do as he liked 

 — he broke his articles — became a medical student in London, but did 

 not complete his course ; he drifted finally to Switzerland and attended 



