518 METHYLEN BLUE 



They are then quickly washed and treated for a few minutes 

 with 1 per cent, gold chloride, quickly washed once more, and 

 reduced in 1 per cent, watery solution of anilin oil or resorcin. 

 Dehydrate and mount in balsam. 



1051. Lennhoff's Processes (Neurol. Cenfrbl., xxix, 1910, p. 20). 

 (1) Polychrome-methylen blue and potassium sulphocyanide method for 

 axis-cylinders : Fixation not stated. Stain sections in polychrome 

 methylen blue for two to five minutes, wash them in distilled water and 

 transfer them for half to twenty-four hours into potassium sulpho- 

 cyanide (strength not stated). Wash, dehydrate, clear, and mount in 

 balsam. (2) Polychrome-methylen blue and potassium ferricyanide 

 method for axis-cylinders and nerve-cells : Sections of material fixed 

 in alcohol are treated as above, using potassium ferricyanide instead of 

 the sulphocyanide. (3) Iron method : Sections are kept for thirty 

 seconds in 2 c.c. of a 15 per cent, solution of tannin to which 3 drops of 

 a 5 per cent, solution of oxalic acid have been added. Rinse them first 

 in distilled water and then for a few seconds in 1 per cent, solution of 

 iron chloride until no further blackening occurs. Wash, dehydrate and 

 mount in balsam. Axis-cylinders black, nerve-cells grey. 



Apathy's Gold Method. See § 410. 



Gerlach's Bichromate and Gold Process. See § 408. 



Ramon y Cajal's Gold Method. See Rev. trim. Micr., v, 1900, p. 95. 



Upson's Gold and Iron and Vanadium Methods. See Mercier, 

 Ztschr. wiss. Mikr., vii, 1891, p. 474. 



Magini's Zinc Chloride Process. See Boll. Ace. med. Roma, 1886, or 

 Ztschr. wiss Mikr., v, 1888, p. 87. 



Monti's Copper Process. See Rend. R. Ace. Lincei, Roma, v, 1889, 

 p. 705. 



Strahuber's Anilin Blue Method. See Centrbl. allg. Path., xii, 1901, 

 p. 422. 



Chilesotti's Carmine Stain. See Centrbl. allg. Path., xii, 1902, 

 p. 191 ; Ztschr. zviss Mikr., xix, 1902, p. 161, and xx, 1903, p. 87. 



Kaplan's Anthracen Ink Method. See Arch. Psych., xxxv, 1902, 

 p. 825. 



Mallory's Phosphomolybdic Haematoxylin. See § 311. 



DoNAGGio's Tin Stain. See § 313. 



METHYLEN BLUE METHODS NOT CONSIDERED IN 



CHAPTER XVI. 



1052. S, Meyer's Method for the Central Nervous System 



{Arch. mikr. Anat., xlvi, 1895, p. 282, and xlvii, 1896, p. 734). 

 The method consists essentially in injecting animals sub- 

 cutaneously with large quantities of a solution of methylen 

 blue B.X., and in treating the central organs (brains) with Bethe's 

 fixing bath. S. Meyer used, at first, a 1 per cent, solution ; later, 

 a solution of methylen blue B.X. saturated at the body tem- 

 perature of the animal to be injected (viz., about 5 to 6 per cent.). 

 The injections are to be made at short intervals and in such a way 

 that the animal receives the total quantity it can support in 

 about one to two hours. A cat can support even 150 c.c. ; half- 

 grown rabbits, 30 to 50 c.c. ; fully-developed guinea-pigs, 30 to 



