520 NEUROKERATIN 



Cox {Anat. Hefte, i, 1898, p. 102, note) fixes nerves in 2 per 

 cent, osmic acid (rabbit) or 1 per cent, (frog), washes, dehydrates, 

 clears with bergamot oil, and mounts in balsam. The bergamot 

 oil dissolves out the myelin, and leaves the neurokeratin visible. 

 It may be necessary to leave the nerves for forty-eight hours in 

 the oil. 



Corning {Anat. Anz., xvii, 1900, p. 309) studies the neuro- 

 keratin network in the sciatic of the frog by means of sections 

 of sublimate material strongly stained with iron haematoxylin. 



Kaplan (Arch. Psychiatr., xxxv, 1902, p. 825) stains sections 

 with acid fuchsin and differentiates them by Pal's method. 



Gedoelst {La Cellule, v, 1889, p. 136) has the following : 

 {a) A nerve is treated with liquid of Perenyi, either pure or with 

 addition of a trace of osmic acid, and examined in glycerin. By 

 this treatment the myelin loses its excessive refractivity and 

 the neurokeratin network comes out clearly, {b) Silver nitrate. 

 Good images, but uncertain, (c) Treatment with a mixture of 

 1 per cent, osmic acid and absolute alcohol. The network comes 

 out black. 



