526 MYELIN STAINS 



Pavlow (ibid., xxi, 1904, p. 14) uses the permanganate twice as 

 strong as Pal. 



KozowsKY {Neurol. Centrbl., xxiii, 1904, p. 1041) stains as Weigert, 

 and differentiates the sections first with 1 per cent, permanganate, till 

 the grey matter comes out brown, and finishes the differentiation 

 with Liq. Jerri sesquichlorati. 



Potter (Ztschr. wiss. Mikr., xxvii, 1910, p. 238) fixes in 10 per cent, 

 formalin, cuts slabs 15 mm. thick, and mordants these for fourteen days 

 in Weigert's gliabeize, washing out in increasing strengths of alcohol 

 and imbedding in celloidin. Thin sections, down to 10 y., are stained 

 for two and a half to three hours in Weigert's iron haematoxylin, made 

 without acid, washed in distilled water, and differentiated, first in 

 J per cent. pot. permanganate, and then in borax-ferricyanide solution. 



Kaiser (Neurol. Centrbl., xii, 1893, p. 364), Bolton (Journ. 

 Anat. and Phys., xxxii, 1898, p. 247), Wynn {ibid., xxxiv, 1900, 

 p. 381) and Laslett (Lancet, 1898, p. 321) use osmic acid either 

 as a 1 per cent, solution or as Marchi's fluid as a mordant. Bolton 

 also tried 2 per cent, ferric chloride, 2 per cent, iron alum, and 

 ammonium molybdate as primary mordants on frozen sections 

 with good results. For details see early editions. 



1059. Kultschitzky's Method (Anat. Anz., iv, 1889. p. 223 ; 

 and V, 1890, p. 519). Specimens are hardened for one or two 

 months in Erlicki's fluid, imbedded in celloidin or photoxylin, 

 and cut. Sections are stained for from one to three hours, or 

 as much as twenty-four, in a stain made by adding 1 grm. of 

 haematoxylin dissolved in a little alcohol to 100 c.c. of 2 per 

 cent, acetic acid. They are washed out in saturated solution of 

 lithium or sodium carbonate. Dijferentiatioti is not necessary, 

 but by adding to the lithium carbonate solution 10 per cent, of 

 a 1 per cent, solution of potassium ferri-cyanide and decolourising 

 therein for two or three hours or more, a sharper stain is obtained. 

 After this the sections are well washed in water and mounted in 

 balsam. Myelin dark blue. 



WoLTERS (Ztschr. wiss. Mikr., vii, 1890, p. 466) proceeds as 

 Kultschitzky, except that he stains at 45° C. for twenty-four 

 hours in 2 per cent, hsematoxylin with 2 per cent, acetic acid, 

 after which the sections are dipped in Midler's fluid, and 

 differentiated by Pal's method. 



Kaes (Neurol. Centrbl., x, 1891, p. 456) stains in Kultschitzky's 

 haematoxylin for two to three days at 45° C, and differentiates 

 the sections by Pal's method in a porcelain dish, the bottom of 

 which is perforated with fine holes. 



Wolters' is now the standard " Weigert-Pal " method. It is 

 usual to use material which has been fixed in formalin for not 

 less than ten days, slices of which ^ to |^ cm. thick are placed 

 direct in the mordant. 



Perdrau transfers the sections after staining to a bowl of 

 distilled water to which about 2 c.c. of a saturated solution of 



