MYELIN STAINS 527 



lithium carbonate have been added. They are stirred about several 

 times and transferred into a fresh bath of the same solution if 

 necessary, until the celloidin is all but colourless. He lastly 

 differentiates, as by Pal's method (§ 1058), washes, and counter- 

 stains either in alum carmine for ordinary work or in an alcoholic 

 solution of eosin if the preparations are to be photographed. 



1060. MiTROPHANOw {Ztschr. iviss. Mikr., xiii, 1896, p. 470) mordants 

 photoxylin sections for at least twenty-four hours at 40° C. in a mixture 

 of equal parts of saturated aqueous solution of copper acetate and 90 

 per cent, alcohol, stains for ten minutes in Kultschitzky's haematoxy- 

 lin, and differentiates with Weigert's ferricyanide fluid. 



1061. Anderson {Laboratory Journal, v, 1922, p. 65, and 

 Stiffs " Pract. Bact. Bloodwork and Parasit," 7th ed., London, 

 1923, p. 630) has modified Kultschitzky's method for frozen 

 sections. He mordants sections for forty-eight to seventy-two 

 hours at 37° C. in 90 c.e. of Weigert's fluorchrome-bichromate 

 mordant with 10 c.c. of 2 per cent, calcium hypochlorite ; transfers 

 directly to Weigert's copper mordant for ten to thirty minutes, 

 washes and stains for one hour at 50° C. in hsematoxylin freshly 

 made up in the following way. (Mix \ grm. haematoxylin crystals 

 in 10 c.c. of absolute alcohol, add 3 c.c. of 2 per cent, calcium 

 hypochlorite, distilled water to 100 c.c. and then 3 c.c. of glacial 

 acetic acid.) He transfers directly to Miiller's fluid for ten to 

 twenty minutes, washes well, and differentiates by Pal's method. 



1062. Berkley's Rapid Method {Neurol. CentrhL, xi, 1892, p. 270). 

 Slices of tissue of not more than 2| mm. in thickness are hardened for 

 twenty-four to thirty hours in Flemming's fluid, at a temperature of 

 25° C, then in absolute alcohol, then imbedded in celloidin and cut. 

 After washing in water the sections are put overnight into a saturated 

 solution of copper acetate (or simply warmed therein to 35° to 40° C. for 

 half an hour). They are then washed and stained for fifteen to twenty 

 minutes in a lithium carbonate ha;matoxylin similar to Weigert's, 

 warmed to 40° C, allowed to cool, and differentiated for one to three 

 minutes in Weigert's ferricyanide liquid, which may be diluted if 

 desired with one-third of water. 



1063. Hill {Phil. Trans., 184, b, 1894, p. 899) stains well-washed 

 Miiller material in bulk in alum curmiuc, cuts and mordants sections for 

 twenty-four hours in half-saturated solution of copper acetate, stains 

 and differentiates as Weigert, taking the differentiating fluid only half 

 as strong. 



1064. Streeter {Arch. mik. Anat., Ixii, 1903, p. 734) stains small 

 nerve-centres in bulk (after mordanting in Weigert's bichromate and 

 fluoride mixture) with Weigert's lithium carbonate haematoxylin (four 

 to six days), washes for a couple of days in 70 per cent, alcohol, makes 

 paraffin sections, and differentiates them by the method of Weigert or 

 Pal. 



1065. Besta's Ammonio-Chloride of Tin Methods {Riv. Sperim. 

 Freniatr., xxxi, 1905, p. 509). Pieces of peripheral nerves arc 

 fixed for one to three days in 100 c.c. of water with 25 of formol, 



