528 MYELIN STAINS 



and 4 grm. of Merck's ammonio-chloride of tin, and then dehy- 

 drated and imbedded as usual. The sections may be stained in 

 different ways : (a) For twenty-four hours in Mallory's phospho- 

 molybdic-carboHc-acid hsematoxylin with subsequent differentia- 

 tion in Lugol's solution ; (b) for thirty to sixty minutes in a very 

 diluted solution of Delafield's hsematoxylin and then for a minute 

 in Held's acetic solution of erythrosin ; (c) for five to ten minutes 

 in erythrosin, and then for two hours in a mixture of equal parts 

 of 1 per cent, hsematoxylin and 4 per cent, ammonium molybdate 

 with 3 drops of acetic acid to every 50 c.c. of the mixture. 



1066. Gallein. Aronson (Centrbl. med. Wiss., xxviii, 1890, p. 577) 

 stains sections of material, hardened in hquid of Erlicki or Miiller and 

 mordanted with copper acetate, for twelve to twenty-four hours in a 

 solution of 3 to 4 c.c. oi gallein in 100 c.c. of water with 20 of alcohol 

 and 3 drops of a concentrated solution of sodium carbonate. Sections 

 are differentiated by the method of Weigert, or Pal. Nerve-fibres red. 

 A second stain with methylen blue may follow (best after differentiat- 

 ing with potassium permanganate). Similarly Schrotter (Centrbl. 

 allg. Path., xiii, 1902, p. 299). 



1067. Schrotter (Neurol. Centrbl., xxi, 1902, p. 338) also stains 

 sections for two to three hours in a 5 per cent, solution of sodium 

 sulphalizarinate, to which a few drops of 5 per cent, oxalic acid (enough 

 to give an orange tint) are added, then differentiates until no more 

 colour comes away in sodium carbonate solution of xtfVff strength, and 

 mounts in balsam. Myelin red, on a colourless ground. 



1068. Toluidine Blue and Methylen Blue. Harris (Philadelphia 

 Med. Journ., i, 1898, p. 897) stains sections (of material hardened as for 

 Weigert's stain) for several hours in a 1 per cent, solution of toluidine 

 blue in 1 per cent, borax solution, and differentiates in saturated aqueous 

 solution of tannic acid. Similarly, but with methylen blue, in a compli- 

 cated way Fraenkel, (Neurol. Centrbl., xxii, 1903, p. 766). 



BiNG and Ellermann (Arch. Anat. Phys., Phys. Abth., 1901, p. 260) 

 harden in 9 parts of acetone to 1 of formol, cut without imbedding, 

 stain for five to ten minutes in saturated methylen blue solution, and 

 put for one or two into saturated solution of picric acid. 



1069. Other Modifications or Similar Methods. Flechsig, Arch. 

 Anat. Phys., Phys. Abth., 1889, p. 537 ; Breglia, Ztschr. wiss. Mikr., 

 vii, 1890, p. 236 ; Rossi, ibid., vi, 1889, p. 182 ; Mercier, ibid., vii, 

 1891, p. 480 ; Haug, ibid., p. 153 ; Walsem, ibid., xi, 1894, p. 236. 



Strong (Journ. Comjj. Netir., xiii, 1903, p. 291) finds copper bichro- 

 mate (of 2 to 3 per cent.) the best mordant ; and that the mordanting is 

 best done before bringing into cello idin. After staining, he treats for 

 half a minute with 0-25 per cent, osmic acid and differentiates as Pal. 



K. Koch (Berl. Klin. Wochenschr., li, 1914, p. 422) makes sections by 

 the freezing method of formalin material imbedded in gelatin, and 

 after staining with Weigert's iron hjematoxjiin, differentiates by Pal's 

 method, and mounts in glycerin jelly. 



1070. Staining Normal Medullary Sheaths by Osmic Add 



(ExNER, Sitzb. Akad. Wiss. Wien., Ixxxiii, 1881, Abth. 3, p. 151 ; 

 Bevan Lewis, The Human Brain, 1882, p. 105). A portion of 

 brain, not exceeding a cubic centimetre in size, is j^laced in 1 per 

 cent, osmic acid, and after five to ten days is cut (best without 



