530 MYELIN STAINS 



(paraffin or celloidin) for a few minutes with 2 per cent, osmic acid, and 

 reduces in 5 per cent, pyrogallol. 



1072. Iron. Allerhand (Neurol. Centrbl., xvi, 1897, p. 727) puts 

 sections of Miiller material for fifteen minutes into warm 50 per cent, 

 solution of Liquor ferri sesquichlorati, then for an hour or two into 20 

 per cent, tannin solution (old and brown). They are then differentiated 

 by Pal's method, using, however, the liquids twice as strong. 



An iron-alxun process is described by Strong in Journ. Comp. Neurol., 

 xiii, 1903, p. 291. 



1073. Silver Nitrate. Vestarini-Cresi (Att. Accad. Med. Chir. 

 Napoli, 1, 1896) hardens in formol, cuts thick sections, washes them with 

 40 per cent, alcohol, puts them in the dark into 1 per cent, solution of 

 silver nitrate in 40 to 70 per cent, alcohol, then washes thoroughly. 



Similarly, Mosse (Arch. mik. Anal., lix, 1902, p. 401), impregnating 

 bichromic material with 1 per cent, solution of argentamin, and reducing 

 in 10 per cent, pyrogallic acid, and differentiating by the method of Pal. 



MYELIN AND AXIS-CYLINDER STAINS 



1074. Methylen Blue. Sahli (Ztschr. wiss. Mikr., ii, 1885, p. 1) 

 stains sections of tissue hardened in bichromate for several hours, in 

 concentrated aqueous solution of methylen blue, rinses with water, and 

 stains for five minutes in saturated aqueous solution of acid fuchsin. 

 If now the sections are rinsed first with water, then for a few seconds in 

 a 1 : 1000 alcoholic solution of caustic potash, and lastly brought into a 

 large quantity of water, the stain becomes differentiated, axis-cylinders 

 being shown coloured red and the myelin sheaths blue. 



Or (ibid., p. 49), the sections are stained for a few minutes or hours 

 in : — 



Water 40 parts. 



Saturated aqueous solution of methylen blue . 24 „ 

 5 per cent, solution of borax . . . 16 ,, 



then washed either in water or alcohol until the grey matter is distinctly 

 differentiated from the white substance, cleared with cedar-wood oil, 

 and mounted in balsam. Preparations similar to those obtainable by 

 Weigert's method. 



1075. Acid Fuchsin. Finotti (Virchow's Archiv., cxliii, 1896, p. 133) 

 stains strongly in Delafield's hsematoxylin, then for a few seconds in 

 concentrated solution of picric acid, then in 0-5 per cent, acid fuchsin, 

 and treats lastly with alkaline alcohol. 



Ohlmaciier (Journ. Exper. Med., ii, 1897, p. 675) stains sections for 

 one minute with gentian violet in anilin water, then for a few seconds in 

 a 0-5 per cent, solution of acid fuchsin in saturated solution of picric 

 acid diluted with 1 volume of water, and differentiates with alcohol and 

 clove oil. 



Kaplan (Arch. Psychiatr., xxxv, 1902, p. 825) mordants for months 

 in Miiller, stains sections for a day or more in ^ per cent, aqueous 

 solution of acid fuchsin, rinses in water acidulated with HCl, and 

 differentiates by the method of Pal. 



1076. Safranin. Ada:\ikiewicz (Sitzb. Akod. Wiss. Wien. Math. 

 Natunv. Kl., Ixxxix, 1884, Abth. 3, p. 245) stains sections of Miiller 

 material in concentrated solution of safranin, differentiates in alcohol 

 and clove oil, brings back again into water, washes in water acidified 

 with acetic acid, and stains in methylen blue. Myelin red, nuclei violet. 



Similarly, Ciaglinski (Ztschr. iviss. Mikr., viii, 1891, p. 19) and 

 Stroebe (ibid., x, 1893, p. 384), the former employing safranin fol- 

 lowed by anilin blue, whilst the latter first stains with anilin blue, then 



