CHLORIDES ORGANIC ACIDS ETC. 



59 



Carothers' fluid for orthopteran chromosomes. 



See also " Cytology " sections, §§ 625, 1355. 



MoREAtrx [Bibl. Anal., 1910, p. 265) takes 15 parts formol, 85 of 

 trichlor-acetic acid of 3 per cent., and picric acid to saturation. 



116. Picro-platinic Formol (M. and P. BouiN, Bibl. Anat., 1898, 

 f. 2, p. 2). 



Platinum chloride, 1 per cent. sol. . . 20 parts. 



Picric acid, saturated sol. . . . 20 ,, 



Formol ...... 10 ,, 



Formic or acetic acid . . . . 5 ,, 



The mixture does not keep more than a day or two, and it is probably 

 inferior. 



BouiN also {Arch. Biol., xvii, 1900, p. 211) simply substitutes formol 

 for the osmic acid in Hermann's mixture, § 51. 



117. Sublimate Formol (M. and P. Bouin, loc. cit.). A similar 

 mixture, in which sublimate of 1 per cent, is substituted for the platinum 

 chloride. 



Another formula of the same authors {Arch. Biol., xvii, 1900, p. 211) 

 is 1 part of formol to 3 of saturated aqueous sublimate. Rinse with 

 water and bring into alcohol of 70 per cent. 



Spuler (Encycl. mik. Technik., 1st ed., p. 1280) adds to sublimate of 

 3 per cent, or more 1 per cent, of glacial acetic acid and 10 per cent, of 

 formol. 



Mann {Verh. Anat. Ges., 1898, p. 39) takes for nerve-cells 2| grm. 

 sublimate, 1 grm. picric acid, 5 c.c. formol, and 100 c.c. water, or 

 {Methods, etc., p. 97) for all tissues 2J grm. sublimate, 20 c.c. formol, 

 and 80 c.c. water. 



Branca {Journ. Anat. et Phys., xxxv, 1899, p. 767) adds 10 parts of 

 formol and 1 of acetic acid to 60 parts of saturated solution of picric 

 acid with saturated sublimate. 



NowAK {Anat. Anz., xx, 1901, p. 244) takes 30 parts of saturated 

 sublimate, 30 of 1 per cent, chromic acid, 27 of water, 3 of acetic acid 

 and 10 of formalin. 



118. Formol-Muller. This is the name given by Ortii {Bed. 

 klin. Wochenschr., 1896, No. 13) to a mixture of 1 part of formol 

 with 10 of liquid of Miiller (§ 58). It should be freshly made up. 

 Fix for three hours in the incubator at 37° C, or twelve at normal 



