538 NEUROGLIA AND SENSE ORGANS 



They are then differentiated till clear and light blue with a 

 mixture of equal parts of anilin oil and xylol, washed thoroughly 

 with pure xylol, and mounted in balsam or, preferably, in tur- 

 pentine-colophonium. 



Glia fibres and nuclei blue, cytoplasm stainless. 



This method only gives good results with the human subject. 



Spielmeyer {Tech. der mik. Unter. des Nervensy stems, Berlin, 1924) 

 recommends using a stain composed of 5 parts of a saturated alcoholic 

 solution of methyl violet, 10 parts of absolute alcohol, and 85 parts of 

 5 per cent, phenol. 



1084. Modifications of Weigert's Method. See also Mallory 

 (Joimi. Exper. Med., 1897, p. 532). 



Storch (Virchoiv's Archiv., civil, 1899, p. 127). 



Bartel (Ztschr. zviss. Mikr., xxi, 1904, p. 18). 



Wimmer {Centrbl. allg. Pathol, u. Pathol. Anat., xvii, 1906, p. 566). 



Galesescu (C. jB. Soc. de Biol., Ixv, 1908, p. 429) fixes tissue first for 

 five hours in 6 per cent, sublimate and then for forty-eight hours at 

 37° C. in 3 parts of Fol's modification of Flemming's chromi-osmo- 

 acetic acid with 1 part of 7 per cent, sublimate, changing the fluid two 

 or three times. After washing for two hours in running water the pieces 

 are passed first for twenty-four hours into acetone to which a little 

 tincture of iodine is added, then into pure acetone and imbedded in 

 paraffin. Sections 3 to 4 ^ thick, are put into a 5 per cent, solution of 

 methyl violet 5B, in 80 per cent, alcohol, adding to each 20 c.c. of this 

 stain 1 c.c. of 5 per cent, oxalic acid. Stain first in the cold for ten 

 minutes and then warm gently over the flame for five minutes. Pour 

 on Gram's iodine, heating again slightly, blot thoroughly with filter 

 paper and decolourise with equal parts of xylol and anilin oil. 



Anglade and Morel's Victoria Blue Method {Rev. Neurol., ix, 

 1901, p. 157). Harden the tissue as Galesescu, and stain paraffin 

 sections in a saturated aqueous solution of Victoria blue, heated till it 

 steams ; rinse with Gram's fluid, and differentiate, without washing, 

 in xylol 1 part, anilin 2 parts. Wash well in xylol and mount in 

 balsam. Simple, applicable to lower animals and gives very sharp 

 pictures. 



Lhermitte and Guccione {Semaine Med., xxix, 1909, p. 205) have 

 the following modifications of Anglade and Morel's method. Sections 

 made by the freezing method from formalin material are collected in 

 distilled water and then kept for two hours in a cold saturated solution 

 of sublimate and for two days in a mixture consisting of 3 parts of 

 1 per cent, osmic acid, 35 of 1 per cent, chromic acid, 7 of 2 per cent, 

 acetic acid, 55 of distilled water. 



A slide is then covered with cigarette paper and a section floated on 

 to this out of water, drained almost dry and stained for a few minutes 

 with a 1-5 per cent, watery solution of Victoria blue, heating it gently 

 till steam rises three to five times. Throw off excess of stain and pour 

 on Lugol's iodine (iodine 1, pot. iodide 2, water 200) and leave on one 

 minute. Pour off excess of iodine, pour on equal parts of anilin oil and 

 xylol, and leave on till the heavier masses of stain are removed. Then 

 turn the cigarette paper upside down on to a clean slide, blot firmly, 

 and remove paper, leaving the section on the slide. Continue differen- 

 tiation with anilin oil-xylol, wash thoroughly with xylol. Mount in 

 Canada balsam. 



Similarly De Albertis {Pathologica, xii, 1920, p. 240). 



Spielmeyer {Technik der mik, TJntersuch des Nerven, Berlin, 1924) 

 also gives the following " Heidelberger " method. Cello idin sections 



