NEUROGLIA AND SENSE ORGANS 539 



of formalin, or better alcohol-fixed material arc freed from celloidin and 

 fixed to slides with methyl alcohol and stained for twelve hours in 

 1 per cent. Victoria blue, and further treated with iodine, etc., as in 

 Weiger's method. 



Merzbacher (Journ. Neurol. Psych., 1909, xii, p. 1) treats either 

 frozen or parafBn sections of formalin material or celloidin sections 

 fixed to the side by methyl alcohol for two to five minutes with an 

 alkaline alcoholic solution (absolute alcohol 70, 10 per cent, caustic 

 soda 20, distilled water 10), stains in the cold for twenty-four hours 

 with saturated solution of Victoria blue, boiled for one hour, treats 

 with iodine and differentiates as Weigert. 



Anderson (J. Path. & Bad., xxvi, 1923, p. 431) treats frozen sections 

 of formalin material or paraffin sections of material fixed, or after- 

 hardened, in Bouin's fluid for ten to thirty minutes with a mordant 

 consisting of 1 part of 5 per cent, ferric chloride and 2 parts of the 

 following mixture. (Distilled water, 100 c.c, sod. sulphite 5 grm., 

 oxalic acid 2-5 grm., pot. iodide .5 grm., iodine 2-5 grm., glacial acetic 

 acid 5 c.c.) Wash in distilled water and transfer first to J per cent, 

 permanganate for five minutes and then to freshly-mixed Pal's solution 

 for five minutes or more. The sections should be left in this solution 

 until they can be stained. After a quick wash the sections are floated on 

 an albuminised slide, blotted and almost allowed to dry. They are then 

 stained by pouring on boiling 1-5 per cent. Victoria blue, and kept 

 slightly warm in this stain for five minutes. After pouring off the stain 

 strong liUgol's iodone (iodine 1 grm., pot. iodide 2 grm., distilled water 

 100 c.c.) is dropped on to the middle of the section and allowed to act 

 for one minute. The section is then differentiated with equal parts of 

 anilin oil and xylol for fifteen seconds, and then washed till clear, with 

 xylol, after which it is blotted and further differentiated with xylol and 

 anilin oil, washed thoroughly with xylol, and moimted in Canada 

 balsam. Anderson points out that the anilin oil-xylol mixture is 

 much more active so long as any water is left in the tissue and may 

 easily differentiate the finer fibres too far at this stage, but once the 

 water has been eliminated there is much less danger of over-differen- 

 tiating. 



HOLZER {Zeit. f. d. ges. Neur. u. Psych., Ixix, 1921, p. 354) treats 

 frozen sections of formalin material first for half to one and a half 

 minutes with a mixture of 1 part of | per cent, phosphomolybdic acid 

 and 2 parts of 90 per cent, alcohol, floats them on to a slide out of this 

 solution, blots with filter paper wetted with a mixture of 2 parts absolute 

 alcohol, and 8 parts of chloroform, and stains with a solution of 0-5 grm. 

 crystal violet in 2 c.c. of alcohol and 8 c.c. of chloroform. Wash off the 

 stain with 10 per cent, watery pot. bromide solution. Blot once with 

 filter paper wet with a mixture of 4 c.c. anilin, 6 c.c. chloroform, and 

 1 drop of 1 per cent. HCl, and differentiate further with this solution. 

 Wash thoroughly with xylol and mount in balsam. 



Warkany {Zeitschr. f. Wissenschaft. Mikro., iv, 1924, 1, p. 508), 

 treats frozen sections with equal parts of 1 per cent, phosphomolybdic 

 acid and 95 per cent, alcohol, stains them as Holzer, but differentiates 

 in two changes of anilin oil. 



See also Aguerre, Arch. mik. Anat., Ivi, 1900, p. 509 ; Krause, Abh. 

 k. Akad. Wissench. Berlin. Anhang, 1899. 



Rubasciikin {Arch. mik. Anat., Ixiv, 1904, p. 577) recommends 

 injecting centres of small mammals with the fixing liquid. To make 

 this, take 100 parts of 2-5 per cent, solution of potassium bichromate 

 and 0-5 to 1 of copper acetate, boil, and add 2-5 to 5 of glacial acetic 

 acid. To this (which may be kept in stock) add, just before use, 10 per 

 cent, of formol. Inject warm, and after ten minutes dissect out and 



