560 NEUROGLIA AND SENSE ORGANS 



hitern. Monatsschr. Anat. Phys., xxi, 1905, p. 393, and Trab. Lab. 

 Inv. Biol., XV, 1920, p. 1. 



GoLGi's sublimate impregnation (Cox's form) has also been 

 suceessfully employed by Krause and Ramon y Cajal. 



The bichromate and silver method serves for the study of the 

 fibres of Miiller and neuroglia cells, as well as neurones. Weigert's 

 neuroglia stain does not give good results. 



After Zenker fixation Mallory's phosphotungstic acid haema- 

 toxylin may be used. 



Lennox (Arch.f. Ophthalm., xxxii, 1886, 1 ; Zeit. wiss. Mik., iii, 1886 

 p. 408) has used Weigert's hsematoxylin method. 



KuHNT (Jen. Zeit. Naturw., Bd. xxiv, 1890, p. 177) employs Pal's 

 modification. Similarly Schaffer (Sitzb. Akad. wiss. Wien., xcix, 

 1890, Abth. 3, p. 110 ; Zeit. wiss. Mik., viii, 1891, p. 227). These 

 methods give a differential stain of rods and cones. 



For the zonula and ciliary body see Ma was, Arch. d'Anat. micr., xii, 

 1910, p. 103. 



1103. Dissociation. For maceration preparations you may use 

 weak solutions (0-2 to 0-5 per cent.) of osmic acid for fixation and 

 then macerate in 0-02 per cent, chromic acid (M. Schultze), or in 

 iodised serum (M. Schultze), or in dilute alcohol (Landolt), or 

 in Miiller's solution, or (Ranvier, Traite, p. 957) in pure water, 

 for two or three days. Thin {Journ. of Anat., xiii, 1879, p. 139) 

 obtained very good results by fixing for thirty-six to forty-eight 

 hours in one-third alcohol, or in 25 per cent, alcohol, and then 

 staining and teasing. 



ScHiEFFERDECKER maccratcs fresh retina for several days in 

 the methyl mixture, § 577. 



Krause {Intern. Monatsschr. Anat., i, 1884, p. 225) recommends 

 treatment for several days with 10 per cent, chloral hydrate 

 solution ; the rods and cones are well preserved. 



INNER EAR 



1104. Inner Ear, Dissection. For the dissection of the human ear see 

 PoLiTZER, " Die anatomische u. Iiistologische Zergliederung d. men- 

 schlichen Gehoroganes," Stuttgart (Enke), 1889 {Zeit. wiss. Mik., vii 

 1890, p. 364). Amongst the lower mammalia, the guinea-pig is a 

 favourable subject, as here (as with some other rodents) the cochlea 

 projects freely into the cavity of the bulla, and may be easily removed 

 with a scalpel and brought into a fixing liquid, and opened therein. 

 With fishes and amphibia also the membranous labyrinth may easily 

 be got away. 



1105. Preparation. Schwalbe {Beiir. z. Phys. (C. Ludwig's 

 Festschr.), 1887, p. 200). Fix (cochlea of guinea-pig) for eight to 

 ten hours in " Flemming," wash in water, decalcify (twenty-four 

 hours is enough) in 1 per cent, hydrochloric acid, wash the acid 

 out, dehydrate, and imbed in parallin. 



