562 NEUROGLIA AND SENSE ORGANS 



p. 953) recommends for human material, opening the superior 

 semi-circular canal before fixation so that the fixative shall 

 penetrate to the internal ear. The block, which is trimmed 

 as small as possible, is decalcified, after thorough fixation in 

 5 per cent, formol or Miiller's fluid, by equal parts of 5 per cent, 

 formol and 5 per cent, nitric acid, or in Perenyi's solution, changing 

 the fluid very frequently. At least one month is needed for this. 

 Wash in water four to five days, then pass gradually through 

 the alcohols to alcohol and ether, and imbed in celloidin (thin 

 celloidin one month, using an evacuation pump carefully to get 

 rid of air bubbles from the internal ear, thick celloidin one month). 



1106, Other Methods, Waldeyer, Strieker's Handh., p. 958 (decal- 

 cification either in 0001 per cent, palladium chloride containing 10 per 

 cent, of HCl, or in chromic acid of 0-25 to 1 per cent,). 



Urban Pritchard {Journ. Roy. Mic. Soc, xii, 1872, p, 380). Decal- 

 cification in 1 per cent, nitric acid. 



Lavdowsky {Arch. mik. Anat., xiii, 1877, p, 497), Fresh tissues 

 (from the cochlea) are treated with 1 per cent, solution of silver nitrate, 

 then washed for ten minutes in water containing a few drops of 0-5 or 

 1 per cent, osmie acid solution, and mounted in glycerin. 



Max Flesch (Arch. mik. Anat., xvi, 1879, p, 300) ; Tafani (Arch. 

 Ital. de Biol., vi, 1884, p. 207) ; Eichler (Abh. math-phys. CI. Sachs. 

 Ges. Wiss., xviii, 1892, p, 311 ; Zeit. wiss. Mik., ix, 1892, p. 380 (injec- 

 tion of blood-vessels of the labyrinth) ) ; Siebenmann (Die Blutgefasse 

 im Labyrinthe des menschlichen Ohres, Wiesbaden, Bergmann, 1894 ; 

 Zeit. wiss. Mik., xi, 1894, p. 386 ; Gray (Journ. Anat. Phys., xxxvii, 

 1903, p. 379) ; Scott (ibid., xliii, 1909, p. 329). 



1107. Olfactory Nerve-endings, Tactile Corpuscles, etc. Besides 

 the gold method, Chapter XIX, and the methylen blue rnethod. 

 Chapter XVIII, the rapid bichromate and silver method of Golgi 

 should be employed, and for the olfactory mucosa gives the 

 best results. See van Gehuchten, La Cellule, vi, 1890, p, 405. 

 For intra-epidermic tierve-endings, besides the methods given 

 in Chapter XL, the Golgi method should be employed. 

 According to van Gehuchten {La Cellule, ix, 1893, p. 319) it 

 gives much better results than gold methods. He uses the rapid 

 process. For tactile corpuscles, etc, besides the methods given 

 in Chapter XL, see Ramon y Cajal's neuro-fibril methods. 



