SERIAL SECTION MOUNTING 115 



This method allows of the staining of sections on the slide 

 with perfeet safety, both with alcoholic and aqueous stains, 

 provided they be not alkaline. 



According to Lee's experience, the albumen method is absolutely 

 safe, provided that alkaline fluids be avoided in the after-treatment. 

 It has the defect that certain plasma stains (not chromatin stains) 

 colour the albumen very strongly, and cannot be removed from 

 it, and that sections are not expanded by it. 



It sometimes happens that the mixture after it has stood for 

 some time becomes turbid, and at last coagulates, passing into 

 a caseous state ; or it may undergo a hyaline coagulation, drying 

 up like amber. But up to the very last it does not in general 

 lose its adhesive properties. We have, however, found it to do 

 so, after keeping for five or six years, so that, to be on the safe 

 side, it may be well to make it up fresh every six months. 



Heidenhain (Zeit. wiss. Mikr., xxii, 1905, p. 331) makes it 

 up with 1 grm. of blood albumen dissolved in 25 c.c. of water, 

 and an equal volume of 50 per cent, alcohol. 



210. The Albumen and Water Method (Henneguy, Journ. de 

 VAnat. et de la Physiol., 1891, p. 398). A drop of water is spread 

 on a slide painted with Mayer's white-of-egg mixture, the sections 

 are arranged on it, the whole is warmed {not to the melting-point 

 of the paraffin) until the sections flatten out ; the water is then 

 evaporated off at a temperature of about 40° C, and as soon as 

 it has sufficiently disappeared, which at that temperature will be 

 in about ten to fifteen minutes, the slide is further treated as 

 described last §. 



This is a most valuable method. It is quicker than the water 

 method, and, for difficult material, safer. 



See also Ohlmacher, Journ. Amer. Med. Assoc., April, 1893. 



The so-called " Japanese " method, attributed to Ikeda by Reinke 

 {Zeit. wiss. Mik., xii, 1895, p. 21), is merely that of Henneguy. 



Mann {Anat. Anz., viii, 1893, p. 442) shakes up white of egg with 

 water, coats slides with it and dries them. He flattens sections on 

 water at 40° C, lifts them out on a prepared slide, and dries for five 

 minutes at 35° C. 



211. Garlic-water. Hollande {Arch. d'Anat. Micr., xiii, 1911, p. 

 171) gives the following as more adhesive than albumen : — 50 grm. of 

 crushed and chopped garlic are rubbed up with 80 c.c. of chloroform- 

 water (Codex, A.C.) and filtered after twenty-four hours. Use as 

 albumen. 



212. Schallibaum's Collodion {Arch. mikr. Anat., xxii, 1883, p. 565). 

 One part of collodion shaken up with 3 — 4 parts of clove or lavender 

 oil. Use as albumen. Sections can be treated with alcohol (not 

 absolute) and divers staining fluids. Lee did not find it safe for this. 

 Rabl, however {Zeit. wiss. Mik., xi, 1894, p. 170), finds that it is if you 

 take 2 parts of collodion to 3 of clove oil, and make up fresh every four 

 or five days. 



213. Obregia's Method for Paraffin or Celloidin Sections 

 {Neurologisches Centralb., ix, 1890, p. 295 ; Gulland, Jowrn. of Path., 



