SERIAL SECTION MOUNTING 117 



Similarly, Ruppricht, loc. cit, last §, with the needless com- 

 plication of a seriation on Strasser's collodionised paper. 



Strasser (loc. cit., last §) also employs a dry gelatin film which 

 he makes sticky by means of carbol-xylol. 



METHODS FOR CELLOIDIN SECTIONS 



216. The Albumen Method. Lee finds that celloidin sections 

 may be mounted on Mayer's albumen, and have the celloidin 

 removed, if desired, by putting them into ether-alcohol. Care 

 must be taken to press them down very thoroughly on to the 

 albumen ; and it is well not to have them too wet. 



Similarly, Jordan (Zeit. wiss. Mik., xv, 1898, p. 54), and 

 Argutinsky {ibid., xvii, 1900, p. 37). See also Jordan, ibid., 

 192—194 ; Dantschakoff, ibid., xxv, 1908, p. 35 ; Maximow, 

 ibid., xxvi, 1909, p. 184 ; Anitschkow, ibid., xxvii, 1910, p. 68 ; 

 Weber, ibid., xxix, 1912, p. 186 ; Rubaschkin, Anat. Anz., 

 xxxi, 1907, p. 30. Weber paints over the series on the albumen 

 with a layer of thin collodion, and puts into alcohol of 50 per 

 cent., then into a mixture of equal parts of chloroform and 

 absolute alcohol. After staining, pure absolute alcohol must be 

 avoided. 



217. Summers' Ether Method (Amer. Mon. Mic. Journ., 1887, p. 73). 

 Place the sections in 95 per cent, alcohol for a minute or two, arrange 

 on the slide, and then pour over the sections sulphuric ether vapour, 

 from a bottle partly full of liquid ether. The celloidin will immediately 

 soften and become perfectly transparent. Place the slide in 80 per 

 cent, alcohol, or even directly in 95 per cent, if desired. We have not 

 ourselves found this method safe. 



Instead of pouring the ether vapour over the slide, it may, of course, 

 be treated with ether vapour in a preparation glass or similar arrange- 

 ment, which Lee thinks preferable. 



Gage (Proc. Amer. Soc. Mic., 1892, p. 82) advises that the slide 

 be one that has been previously coated with a 0-5 per cent, solution 

 of white of egg and dried ; the collodion adheres much more strongly 

 to an albuminised surface. 



AuBURTiN [Anat. Anz., xiii, 1897, p. 90) arranges on a clean slide, 

 dehydrates the sections with blotting-paper and treatment with absolute 

 alcohol, then drops on to them a mixture of alcohol and ether which 

 dissolves out the celloidin from the sections, then allows the thin 

 collodion thus formed to evaporate into a thin sheet on the slide. Then 

 70 per cent, alcohol and other desired reagents. 



Similarly, Maier (Munch, med. Wochenschr., Ivii, 1910, No. 12 ; Zeit. 

 wiss. Mik., xxvii, 1910, p. 385), but adding a treatment for ten to fifteen 

 minutes with bisulphide of carbon. 



See also Myers, Arch. Anat. Phys., Anat., Ahth., 1902, p. 371 (com- 

 plicated). 



218. Apathy's Oil of Bergamot Method {Mitth. Zool. Stat. 

 Neapel, 1887, p. 742 ; Zeit. wiss. Mile, v, 1888, pp. 46 and 360, 

 and vi, 1889, p. 167). Cut with a knife smeared with yellow 

 vaseline and wetted with 95 per cent, alcohol. Float the sections, 



