SERIAL SECTION MOUNTING 119 



The plate may now be either put away till wanted in 80 per 

 cent, alcohol, or may be brought into a staining fluid. The 

 watery fluid causes the double sheet of collodion to become 

 detached from the glass, holding the sections fast between its 

 folds. It is then easy to stain, wash, dehydrate, and mount in 

 the usual way, merely taking care not to use alcohol of more 

 than 90 to 96 per cent, for dehydration. Weigert recommends 

 for clearing the mixture of xylol and carbolic acid (3:1). 



The series should be cut into the desired lengths for mounting 

 whilst in the alcohol. 



A good method for large and thick sections. 



For Blochman's modification see § 213. 



Strasser takes gummed paper instead of the glass plates used in this 

 process. See the papers quoted § 214. 



See also Wintersteiner {Zeit. iviss. Mik., x, 1893, p. 316) and Kubo 

 {Arch. mik. Anat., Ixx, 1907, p. 173). 



221. Obregia's Method. Slides are prepared as directed 

 (§ 213), the sections are arranged on them and covered with 

 celloidin or photoxylin and evaporated as described, § 213. 



For Dimmer's modification see also § 213. 



222. CoUodion Film Method. Graham Kerr {in Hit.. 1908) 

 seriates on Kodak films. A film has the emulsion removed by 

 hot water. The sections are arranged on a dry film, and the 

 application of a drop of absolute alcohol and ether (or an atmo- 

 sphere of alcohol and ether) suffices to weld them into a mass 

 with the film. The sheet may then be stained and mounted or 

 rolled up and stored in cedar oil. 



The late Dr. S. G. Scott used mica sheets, upon which he stuck 

 paraffin sections. These could be distributed to a class of students by 

 simply cutting out pieces of mica supporting the sections. 



Other Methods for Celloidin Sections. See § 215 (Fol) and 

 § 227 (Olt). 



