INVERTEBRATES 591 



with 1 of formol and 9 of sea-water, Doliolidit with svibhmate, 

 or the above osmic mixture, or a mixture of 10 parts 10 per eent. 

 solution of sulphate of copper with 1 part concentrated subhmate 

 sokition, or the formol mixture. 



MOLLUSCOIDA 



1168. Bryozoa. For some methods of killing and fixing see 

 §§ 10 and 27. S. Lo Bianco employs for PedicelUna and 

 Loxosoma the chloral hydrate method, fixing with sublimate. 

 For Flusira, Celleporo, Bugulo, Zooboihrium, he employs the 

 alcohol method of EisiG, § 17. For Cristetella see §§ 11, 19. See 

 also Braux. 



CoNSER {Trans. Amer. Mic. Soc, xvii, 1896, p. 310) kills the 

 fresh-water forms with cocaine, puts them for an hour into 1 per 

 cent, chromic acid, and passes through water into alcohol, etc. 



Similarly Calvet {Hist. Nat. Bryozoaires, Montpellier, 1900, 

 p. 15), for marine forms. 



ZscHiESCHE {Zool. Jahtb., xxviii, 1909, p. 6) fixes larvae of 

 Alcyonidium (settled down on a layer of celloidin or paraffin) 

 with 8 parts of sublimate and 2 of acetic acid to 90 of sea-water, 

 for twenty-five to thirty minutes. 



1169. Brachiopoda. Lo Bianco kills small animals in 70 per 

 cent, alcohol, larger ones being first narcotised with alcohol and 

 sea-water. 



Blochmann {Untersuch. fein. Bau Brachiopoden, Jena, 1892, 

 p. 5) fixes principally with sublimate, macerates by the Hert- 

 wiGs' method, § 568, decalcifies with 1 per cent, chromic acid 

 (for thick shells add a little hydrochloric or nitric acid), or with 

 nitric acid in alcohol of 50 to 70 per cent., and imbeds in paraffin 

 or celloidin. 



See also FiKMAN, Zeit. wiss. Zool., Ixii, 1896, p. 172. 



MOLLUSCA 



1170. Fixation. To kill Mollusca extended for dissection 

 make up stock solution A : 90 parts absolute alcohol, 10 parts 

 turpentine ; 10 per cent, of A to 90 per eent. water. Leave in 

 twelve to twenty-four hours. Lo Bianco narcotises Lamelli- 

 branchs for six to ten hours or more with alcohol, § 17, and then 

 kills them. 



List {Fauna Flora Golf. Neapel, xxvii, 1902, p. 292) narco- 

 tises Mytilidte with 2 per cent, of cocaine in sea-water, and (for 

 preservation of cilia) fixes in sea-water, with 10 per cent, of 

 formol, 



Lo Bianco advises that Prosobranchiata, and, amongst the 

 Heteropoda, Atlantidae, be narcotised with 70 per cent, alcohol, 



