594 INVERTEBRATES 



1177. Injection of Acephala (Flemming, Arch. mik. Anat., 

 1878, p. 252). To kill the animals freeze them in a salt and ice 

 mixture, and throw them for half an hour into lukewarm water. 

 They will be found dead, and the injection-pipe may be tied in 

 the hea'rt, and the entire animal filled and covered up with 

 plaster of Paris, which serves to occlude cut vessels that it is not 

 possible to tie. As soon as the plaster has hardened the injec- 

 tion may be proceeded v.ith. See also Dewitz, Anleit. zur 

 Anfert. zootom. Prdp., Berlin, 1886, p. 44 (AnodoiHa) and p. 52 

 (Helix). 



Dakin {Liverpool Alar. Biol. Comni., xvii, 1909, p. 76) narco- 

 tises by adding alcohol and glycerin for eighteen to twenty-four 

 hours, puts for half an hour into formol of 5 per cent,, and injects 

 into a branchial vessel. ' 



MozEJKO {Zeit. wiss. Mik., xxvi, 1909, p. 353, and 1910, p. 542) 

 puts for half an hour into water at 40" to 50° C, removes the 

 shell, and injects carmine through the heart. For occluding vessels 

 he takes cotton-wool soaked with gelatin and plaster of Paris. He 

 takes for a vaso-dilator a saturated solution of peptonmn siccum. 



1178. Maceration Methods for Epithelium. Engelmann 

 {PfliXger's Arch., xxiii, 1880, p. 505) macerates the intestine of 

 Cyclas in osmic acid of 0-2 per cent, (after having warmed the 

 animal for a short time to 45° to 50° C), or in concentrated 

 boracic acid solution. 



Cilia. The entire intra-cellular libre apparatus may be isolated 

 by teasing fresh epithelium from the intestine of a Lamellibranch 

 {e.g., Anodonta) in either bichromate of potash of 4 per cent, 

 or salt solution of 10 per cent. To get good views of the apparatus 

 in situ in the body of the cell, macerate for not more than an 

 hour in concentrated solution of boracic or salicylic acid. Very 

 dilute osmic acid {e.g., 0-1 per cent.) gives also good results. 

 The " lateral cells " of the gills are best treated with strong 

 boracic acid solution (5 parts cold saturated aqueous solution 

 to 1 part water). 



Dr. Orton uses borax carmine and picro-nigrosin {in litera). 



Bela Haller's Mixture, see § 574 ; Brock's Medium, § 565 ; 

 MoBius's Media, § 569 ; the second of these is much recom- 

 mended by Drost {Morphol. Jahrb., xii, 1866, p. 163) for Cardium 

 and My a. 



Patten {Mitth. Zool. Stat. Neapel, vi, 1886, p. 736) takes 

 sulphuric acid, 40 drops to 50 grm. of water. Entire molluscs, 

 without the shell, may be kept in it for months. 



Bernard {Ann. Sci. Nat. ix, 1890, p. 191) macerates the 

 mantle of Prosobranchs in a mixture of 1 part each of glycerin 

 and acetic acid, 2 parts each of 90 per cent, alcohol and 0-1 per 

 cent, chromic acid and 40 parts water, which acts in from a 

 quarter of an hour to three hours. He also (pp. 102, 306) uses 



