IN VERTEBRA TE8 60 1 



Dr. Orton writes to us that he simply uses picro-nigrosin and borax 

 carmine. 



1190. Tracheae may be studied by the Golgi biehromate and 

 silver process. Martix (C. R. Soc. Philomath., 1893, p. 3) injects 

 them with indigo xvhite (through the body cayity), and puts into 

 hot water from which the air has been expelled by boiling. Tracheae 

 blue. 



1191. Brain of Bees. Kenyon {Journ. Comp. Neurol., vi, 

 1896, p. 137 ; Journ. Roy. Mic. Soc, 1897, p. 80) treats by the 

 Golgi process (seldom successful), or hardens in a mixture of 

 1 part formol and 2 of 5 per cent, sulphate of copper, followed 

 by staining in Mallory's phosphomolybdic hiematoxylin. 



JoNESCU [Jena. Zeit., xlv, 1909, p. Ill) has employed the 

 silver methods of Ramon y Cajal and Bielschowsky and Wolff. 



1192. Ventral Cord. Floyd {Mark. Anniv., 1904, p. 355) fixes 

 the ganglia oiPeriplaneta for eighty minutes with vapour of formol, 

 and brings into alcohol. 



See also Bixet, Journ. Anat. Phys., xxx, 1894, p. 469. 



1193. Eyes of Arthropods. For the methods of Lankester 

 and BouRXE {Quart. Journ. Mic. Sci., 1883, p. 180 : Limulus) ; 

 HiCKSON {ibid., 1885, p. 243 : Musca) ; Parker {Bull. Mus. 

 Harvard Coll., xx, 1890, p. 1 ; Zeit. wiss. Mik., viii, 1891, p. 82 : 

 Homarus) see early editions. 



Parker {Mitth. Zool. Stat. Neapel, xii, 189, p. 1) also applies 

 the methylen blue method to the retina and optic ganglia in 

 Decapods, especially in Astacus. He injects 0-1 c.c. of a 0-2 per 

 cent, solution into the ventral sinus. After twelve to fifteen 

 hours the animals are killed, the ganglia quickly dissected out, 

 and the stain fixed as described, § 382. 



For his method for eyes of Scorpions see § 618. 



For the methods of Purcell for the eyes of Phalangida see 

 Zeit. wiss. Zool., Iviii, 1894, p. 1. He has the following stain. 

 The cephalothorax is removed and brought for twenty minutes 

 into 50 per cent, alcohol warmed to 45° or 50° C, and saturated 

 with picric acid. The pigment dissolves in this solution and 

 stains the nuclei and some other parts of the rhabdoms, so that 

 no further stain is required. 



Hennixgs {Zeit. wiss. Mik., xvii, 1900, p. 326) depigments 

 sections by putting them for ten minutes {Musca) to twelve hours 

 (Myriopoda) into a mixture of 2 parts of 80 per cent, alcohol with 

 1 of glycerin and 2 per cent, of nitric acid, best kept at 35° C. 

 The elements are well preserved. 



WiDMAXX {Zeit. wiss. Zool., xc, 1908, p. 260) makes the lens 

 of Arachnida fit for sectioning by putting for a day or so into 

 alcohol with 10 to 15 per cent, of nitric acid ; and bleaches 

 sections with 1 part of chlorine water to 2 of alcohol. 



