INVERTEBRATES 603 



{Die mik. Techmk, 1885 ; Zeit. zciss. Mik., 1886, p. 61) puts 

 Annelids into a glass cylinder filled with water to the height of 

 10 cm., and then pours 70 per cent, alcohol to a depth of 1 to 

 2 em. on to the water. For Opheliadic he also employs 0-1 per 

 cent, of chloral Indrate in sea-water. 



Many marine Chictopoda may be successfully narcotised (Lo 

 Bianco) in sea-water containing 5 per cent, of alcohol, or by 

 means of the mixture, § 19. 



The Pohjchcvta sedentaria may sometimes be satisfactorily 

 fixed by bringing them rapidly into corrosive sublimate. Cold 

 not hot, solutions should be taken, as heat frequently shrivels 

 up the branehia?. Eunice and Onuphis may be treated in the 

 same way. 



Lo Bianco advises killing Chaetopteridte, Sternaspidic, Spiro- 

 graphis, Protula, by putting them for half an hour into 1 per 

 cent, chromic acid. Some of the sedentaria may be got protruded 

 from their tubes by leaving them for some hours in 0-1 per cent, 

 chloral hydrate in sea-water. 



For Eisig's methods for Capitellidte see Fauna u. Flora Golf. 

 Neapel, xvi, 1887, p. 295. 



See also § 12 (lemon juice), and the methods §§ 10 to 

 27. 



1197. Blood-vessels of Annelids (Kukenthal, Zeit. wiss. Mik., 

 1886, p. 61.) The animals should be laid open and put for two 

 or three hours into aqua regia (4 parts of nitric acid to 2 of hydro- 

 chloric acid). Vessels black, on a yellow ground. 



Bergh {Anat. Hefte, xlv, 1900, p. 392, and xlix, 1900, p. 599) 

 puts small Annelids for a week or more into equal parts of 1 per 

 cent, nitric acid and 1 per cent, nitrate of silver, or into 50 parts 

 of nitrate, 25 of formic acid, and 25 of water, dissects out the 

 organs and exposes to light. Marine forms may be treated by 

 Harmer's process. 



1198. Nerves of Annelids. Note the methylen blue method and 

 the bichromate of silver method of Golgi (the rapid method). For 

 the latter see v. Lenhossek {Arch. mik. Anat., xxxix, p. 102). 



Langdon {Journ. Comp. Neur.,. x, 1900, p. 4) injects strong 

 solution of methylen blue into the body cavity of Nereis, and 

 puts the animal for some hours into sea-water in the dark, fixes 

 the stain by Bethe's method, and makes paraffin sections. 



See also M. Lewis, Abat. Anz., xii, 1896, p. 292 ; Atheson, 

 ibid., xvi, 1899, p. 497 ; and the methods of Apathy, §§ 381 

 et seq. 



1199. Hirudinea. For the methods of killing see those given 

 for Lumbricus in § 1195, also §§ 13 to 26. 



Whitman {Meth. in mic. Anat., p. 27) recommends that they be 

 killed with sublimate. 



Lee has obtained better results by narcotising with carbonic 



