608 INVERTEBRATES 



cent, salt water, and allows them to lie for six to twelve hours or 

 more in moist blotting paper. 



See also Montgomery {Zool. Jahrh., Abth. Morph., x, 1897, p. 6) ; 

 and BoHMiG {Zeii. wiss. Zool., Ixiv, 1898, p. -184). 



1208. Cestodes. Wash gently in 1 per cent, saline, and then 

 fix in hot corrosive sublimate acetic (at circa 50° C.) and allow 

 the tape-worms to remain in the dish till the fluid becomes cold. 

 Wash in running M^ater for twelve hours and transfer to 70 per 

 cent, alcohol. Stain as in general methods. 



As pointed out by Vogt and Yung [Traite d'Anai. Comp. 

 Prat., p. 204), the observation of the living animal may be of 

 service, especially in the study of the excretory system. And, 

 as shown by Pixtxer, Tjcmifc may be preserved alive for several 

 days in common water in which a little white of egg has been 

 added. 



Tower (Zool. Jahrh., xiii, 1899, p. 363) has kept Moniezia 

 expansa alive for several days in a mixture of 100 c.c. of tap- 

 water, 10 grm. of white of egg, 2 of pepsin, 2 of sugar, and 5 of pre- 

 pared beef (" Bovox "). Chloride of sodium, he says, should be 

 avoided. 



LoNNBERG {Centrbl. Bakteriol., xi, 1892, p. 89 ; Journ. Roy. 

 Mic. Sac, 1892, p. 281) has kept Tricenophorus nodidosus alive 

 for a month in a slightly acid pepsin-peptone solution containing 

 from 3 to 4 per cent, of nutritive matter and less than 1 per cent, 

 of NaCl. 



For the nervous system, Tower {Zool. Anz., xix, 1896, p. 323) 

 fixes in a picro-platin-osmic mixture (stronger than that of O. 

 voM Rath, § 106) for ten hours, then treats for several hours with 

 crude pyroligneous acid, and lastly with alcohol, and imbeds in 

 paraffin. 



Zernecke {Zool. Jahrh., Ahth. Anat., ix, 1895, p. 92) kills 

 Ligula in the osmio-bichromic mixture of Golgi (4 : 1), impreg- 

 nates as usual, makes sections in liver, and treats them by the 

 hydroquinone process of Kallius. Besides the peripheral and 

 central nervous system, muscle-fibres, parenchyma cells, and the 

 excretory vascular system are impregnated. 



He has also obtained good results by the methylen-blue 

 method. 



Blochmann {Biol. Centrhl., xv, 1895, p. 14) recommends the 

 bichromate and sublimate method of Golgi. 



See also Koiiler, Zeit. rviss. Zool., Ivii, 1894, p. 386 (stretches Taeniae 

 round a glass plate or on cork, and fixes with 5 per cent, sublimate) ; 

 LuHE, Centrbl. Bakt., xxx, 1901, p. 166, and Ransom, U. S. Nation, 

 Mus. Bull., Ixix, 1909, p. 8. 



1209. Trematodes. If necessary, clean by shaking up in 1 per 

 cent, saline (parasites). Decant off dirty liquid, one-third of the 



