INVERTEBRATES 019 



Zool., Ixvii, 1900, p. 218) fixes young Sycons in absolute alcohol 

 and stains with ammonia carmine (spicules in situ). 



Delage (Arch. Zool. E.rper.. x. 181>2, p. 421) fixes larvic of 

 Spongilla that have settled down on cover-glasses for three 

 minutes in absolute alcohol, stains in alcoholic carmine, and 

 brings through alcohol into oil of bergamot, then cither mounts 

 direct in balsam, or detaches the larvse from the cover and imbeds 

 in paraffin (three minutes). 



Gatenby [Journ. Liniiean Sac, 1920) uses methods for mito- 

 chondria, especially Champy-Kull and Kopsch. 



